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Merck
CN

ABS1004

抗磷酸NKCC1抗体（Thr212/Thr217）

serum, from rabbit

别名:

phospho-NKCC1 Thr212/Thr217, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物来源

rabbit

质量水平

100

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

human

技术

immunofluorescence: suitable
western blot: suitable

NCBI登记号

NP_001037

UniProt登记号

P55011

运输

dry ice

靶向翻译后修饰

phosphorylation (pThr212/pThr217)

基因信息

human ... SLC12A2(6558)

相关类别

一般描述

Na-K-Cl共转运蛋白1（NKCC1）是由SLC12A2基因编码的膜蛋白，在细胞钠/钾/氯化物转运和体内平衡中起作用。它广泛分布于全身并发挥重要作用，特别是在分泌液体的腺体中。NKCC1的细胞内N末端结构域中Thr212/Thr217的磷酸化与NKCC1激活相关。
观察值〜250 kDa。NKCC1可能是NKCC1的成熟形式的二聚体，在不同的细胞中被不同的糖基化，从而导致某些裂解物中的条带未表征。

免疫原

对应于人磷酸-NKCC1 Thr212/Thr217的线性肽。

应用

抗磷酸NKCC1 Thr212/Thr217是用于蛋白质印迹，免疫荧光的针对磷酸NKCC1的抗体。
研究子类别
信号神经科学
研究类别
信号传导
蛋白质印迹分析: 代表性批次在GMB43和GBM5细胞裂解物中检测到磷酸NKCC1 Thr212/Thr217（Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012)JBC.1-31）。
免疫荧光分析: 代表性批次在GBM43人组织中检测到磷酸NKCC1 Thr212/Thr217（Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012)JBC.1-31）。

生化/生理作用

特定于磷酸化NKCC1的成熟形式。

外形

含 0.05％ 叠氮化钠的兔多克隆血清。
未纯化

制备说明

自收到之日起在-20°C可稳定保存1年。
使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏 IgG 和影响产品性能。

分析说明

通过蛋白质印迹对GMB43细胞裂解液进行了评估。

蛋白质印迹分析： 该抗体的1：1,000的稀释液在10 µg GMB43细胞裂解物中检测到磷酸-NKCC1 Thr212/Thr217。

其他说明

浓度：请参考特定批次的数据表。

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

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