Anti-Bak (Ab-2) Mouse mAb (TC-102)

Anti-Bak (Ab-2), mouse monoclonal, clone TC-102, recognizes the ~30 kDa Bak protein in A431, HeLa, MCF-7, and SKBR3 cells. It is validated for Western blotting and immunofluorescence.

Bak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. It was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa, but which migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. Bak is expressed widely in many tissues including heart, thymus, and colon. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA3. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner has a much more extensive tissue distribution, being present in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 kDa (apparent MW) Bak protein.

Recognizes the ~30 kDa (apparent MW) Bak protein in A431, HeLa, MCF-7 or SKBR3 cells.

Epitope: within the N-terminal region

human recombinant Bak protein from which the C-terminal and transmembrane domains have been deleted

Immunoblotting (5 μg/ml)
Immunofluorescence (1 μg/ml)

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Negative Control
SKBR3 cells

Positive Control
A431 cells, HeLa cells

Antibody should be titrated for optimal results in individual systems for each application.