产品名称
Anti-Clathrin Antibody, clone CHC5.9, clone CHC5.9, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
CHC5.9, monoclonal
species reactivity
bovine, amphibian, rat, pig, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CLTC(1213)
Analysis Note
Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Application
Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers
Organelle & Cell Markers
Biochem/physiol Actions
This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Coated vesicles (clathrin) of bovine brain.
Physical form
Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
Preparation Note
Maintain at 2-8°C for up to 12 months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hisako Furukawa et al.
Laboratory investigation; a journal of technical methods and pathology, 84(7), 828-835 (2004-05-04)
Prion protein (PrP) is known to accumulate in some non-neuronal tissues under conditions unrelated to prion diseases. The biochemical and biological nature of such accumulated PrP molecules, however, has not been fully evaluated. In this study, we established experimental myopathy
Yvonne G Y Chan et al.
Cellular microbiology, 11(4), 629-644 (2009-01-13)
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