ChemiKine Nerve Growth Factor, Sandwich ELISA

Research Category
Neuroscience

This ChemiKine Nerve Growth Factor, Sandwich ELISA is used to measure & quantify NGF levels in Neuroscience research.

Used to detect/quantify: NGF

For research use only. Not for use in diagnostic procedures.

With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample.

NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample.ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. The kit utilizes strip well inserts. Running duplicate wells for samples and standards is recommended.

Analytical Sensitivity and Detection Limits:

Sensitivity:10-15 pg/mL

Range of Detection: 10 pg/mL to 500 pg/mL

Crossreactivity: No significant cross reactivity with BDNF, NT4/5 or NT3

Intra-assay Variation:± 4.1% (250 pg/mL)

Inter-assay Variation:± 8.9 % (250 pg/mL)

Test Principle:

With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample.

Application:

ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.

ChemiKine NGF ELISA Plate: (Part No. 90139) Two Pre-coated with Sheep anti-Mouse NGF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.

Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.

Assay Diluent: (Part No. 90143) One 100 mL bottle (Ready to Use).

NGF Standard (Mouse): (Part No. 90142) Two vials (Lyophilized).

Mouse anti-Mouse NGF Monoclonal Antibody: (Part No. 90141) Two vials (Lyophilized).

Donkey anti-Mouse IgG Polyclonal Antibody, HRP conjugated: (Part No. 90140) Two vials (Lyophilized).

TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3′,5,5′-tetramethylbenzidine in a proprietary buffer with enhancer.

Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution (Ready to Use).

192 wells

Precautions:

· Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit′s performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany