ECM555
QCM ECMatrix细胞侵袭试验,96-孔(8 µm),荧光法
The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
别名:
8 μm pore cell invasion assay
质量水平
种属反应性
human
制造商/商品名称
Chemicon®
QCM
技术
activity assay: suitable
cell based assay: suitable
检测方法
fluorometric
运输
wet ice
一般描述
在QCM ECMatrix细胞侵袭检测中,侵袭细胞到达小室底膜,用细胞解离缓冲液处理膜可获取细胞。接着用专利所有的CyQuant GR染料裂解和检测细胞。该染料为绿色荧光染料,可与细胞核酸良好结合,发出强烈的荧光。该检测试剂盒提供了一种通过基底膜模型评估癌细胞侵袭的有效系统。
应用
QCM ECMatrix细胞侵袭检测试剂盒(96孔(8 μm),荧光法)已用于下列细胞的侵袭试验:
- 人宫颈癌细胞
- 骨肉瘤细胞
- 乳癌细胞
- 结直肠癌细胞
特点和优势
- 该检测不需要进行细胞标记、刮取、清洗或计数
- 均匀的荧光检测和96孔小室配置适用于多个样品的大规模筛选和定量比较
- 可快速高效地定量分析多个细胞侵袭相关指标
其他说明
无菌96孔细胞侵袭板组件:(部件号90200)一个96孔进样器托盘和一个带有ECMatrixTM -涂层插入物的96孔细胞侵袭室板。
96孔细胞培养托盘:(部件号90129)一个96孔进样器托盘。
细胞分离溶液:(部件号90131)一瓶 - 16 mL。
4X细胞裂解缓冲液:(部件号90130)一瓶 - 16 mL。
CyQuant GR染料:(部件号90132)一个小瓶 - 75 μL
96孔细胞培养托盘:(部件号90129)一个96孔进样器托盘。
细胞分离溶液:(部件号90131)一瓶 - 16 mL。
4X细胞裂解缓冲液:(部件号90130)一瓶 - 16 mL。
CyQuant GR染料:(部件号90132)一个小瓶 - 75 μL
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
储存分类代码
10 - Combustible liquids
Targeting of Integrin {beta}1 and Kinesin 2{alpha} by MicroRNA 183.
Li G, Luna C, Qiu J, Epstein DL, Gonzalez P
The Journal of Biological Chemistry null
Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels.
Xiao Wang,Carlos T Moraes
Molecular Oncology null
Sheeba Irshad et al.
Cancer research, 77(5), 1083-1096 (2017-01-14)
Cancer cells tend to metastasize first to tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into the lymphatic vasculature remain little understood. Here, we show that in the human breast tumor microenvironment (TME), the presence of increased numbers
Sodium arsenite inhibits migration of extravillous trophoblast cells in vitro.
Chi-Shan Li,Rita Loch-Caruso
Reproductive Toxicology null
Adam S Adler et al.
Nature genetics, 38(4), 421-430 (2006-03-07)
Gene expression signatures encompassing dozens to hundreds of genes have been associated with many important parameters of cancer, but mechanisms of their control are largely unknown. Here we present a method based on genetic linkage that can prospectively identify functional
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持