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Merck
CN

ECM555

Sigma-Aldrich

QCM ECMatrix细胞侵袭试验，96-孔（8 µm），荧光法

The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.

别名:

8 μm pore cell invasion assay

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关于此项目

UNSPSC代码:
12352207
eCl@ss:
32161000
NACRES:
NA.32

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质量水平

100

种属反应性

human

制造商/商品名称

Chemicon®
QCM

技术

activity assay: suitable
cell based assay: suitable

检测方法

fluorometric

运输

wet ice

相关类别

一般描述

在QCM ECMatrix细胞侵袭检测中，侵袭细胞到达小室底膜，用细胞解离缓冲液处理膜可获取细胞。接着用专利所有的CyQuant GR染料裂解和检测细胞。该染料为绿色荧光染料，可与细胞核酸良好结合，发出强烈的荧光。该检测试剂盒提供了一种通过基底膜模型评估癌细胞侵袭的有效系统。

应用

QCM ECMatrix细胞侵袭检测试剂盒（96孔(8 μm)，荧光法）已用于下列细胞的侵袭试验：

  • 人宫颈癌细胞
  • 骨肉瘤细胞
  • 乳癌细胞
  • 结直肠癌细胞
适合用于药物筛选、细胞侵袭相关基质金属蛋白酶(MMP)或蛋白酶检测和转染细胞基因功能分析

特点和优势

  • 该检测不需要进行细胞标记、刮取、清洗或计数
  • 均匀的荧光检测和96孔小室配置适用于多个样品的大规模筛选和定量比较
  • 可快速高效地定量分析多个细胞侵袭相关指标

其他说明

无菌96孔细胞侵袭板组件：（部件号90200）一个96孔进样器托盘和一个带有ECMatrixTM -涂层插入物的96孔细胞侵袭室板。

96孔细胞培养托盘：（部件号90129）一个96孔进样器托盘。

细胞分离溶液：（部件号90131）一瓶 - 16 mL。

4X细胞裂解缓冲液：（部件号90130）一瓶 - 16 mL。

CyQuant GR染料：（部件号90132）一个小瓶 - 75 μL

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

CorrosionEnvironment
GHS05,GHS09

警示用语：

Danger

危险声明

H318,H410

危险分类

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

储存分类代码

10 - Combustible liquids

分析证书(COA)

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Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels.
Xiao Wang,Carlos T Moraes
Molecular Oncology null
Targeting of Integrin {beta}1 and Kinesin 2{alpha} by MicroRNA 183.
Li G, Luna C, Qiu J, Epstein DL, Gonzalez P
The Journal of Biological Chemistry null
Differential expression of matrix metalloproteinases in brain- and bone-seeking clones of metastatic MDA-MB-231 breast cancer cells.
Andreas M Stark,Bartosz Anuszkiewicz,Rolf Mentlein,Toshiyuki Yoneda,H Maximilian Mehdorn et al.
Journal of Neuro-Oncology null
Tumor invasion and metastases: role of the basement membrane. Warner-Lambert Parke-Davis Award lecture.
L A Liotta
The American journal of pathology, 117(3), 339-348 (1984-12-01)
New functions for the matrix metalloproteinases in cancer progression.
Egeblad, Mikala and Werb, Zena
Nature Reviews. Cancer, 2, 161-174 (2002)
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