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Merck
CN

EZBRAIN42

Millipore

Human Amyloid β42 ELISA Kit

measures and quantifies Amyloid β42 levels in 50 μL tissue or cell lysates

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UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.77
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产品名称

Human Amyloid β42 Brain ELISA, This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.

质量水平

种属反应性

human

包装

kit of 1 × 96 wells

参数

50 μL sample volume (Overnight assay)

assay range

sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size)

standard curve range: 16-500 pg/mL

技术

ELISA: suitable

输入

sample type tissue/cellular lysate

应用

research use

检测方法

colorimetric (450nm/590nm)

运输

wet ice

储存温度

2-8°C

基因信息

human ... APP(351)

一般描述

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

应用

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
This assay requires 50 µl of sample and is an overnight assay.
Used to detect/quantify: Amyloid β42

生化/生理作用

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

制备说明

Components in the kit can be stored up to 2 weeks at 2-8°C

其他说明

Please contact Technical Service for linearity of dilution.

免责声明

For research use only. Not for use in diagnostic procedures.

象形图

CorrosionExclamation mark

警示用语:

Warning

危险声明

危险分类

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

储存分类代码

8A - Combustible corrosive hazardous materials

法规信息

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André E S Simões et al.
BMC genomics, 14, 181-181 (2013-03-19)
Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable. TRIzol(®) and TRIzol(®)LS
Y Goll et al.
Neuro-degenerative diseases, 13(2-3), 58-60 (2013-11-07)
Most Alzheimer's disease (AD) cases arise sporadically and may involve innate immune activation of microglial expressed Toll-like receptors regulated through the myeloid differentiation protein 88 (MyD88) pathway. It was the aim of this study to test the innate immune involvement
Jesse E Hanson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(24), 8277-8288 (2014-06-13)
Extensive evidence implicates GluN2B-containing NMDA receptors (GluN2B-NMDARs) in excitotoxic-insult-induced neurodegeneration and amyloid β (Aβ)-induced synaptic dysfunction. Therefore, inhibiting GluN2B-NMDARs would appear to be a potential therapeutic strategy to provide neuroprotection and improve cognitive function in Alzheimer's disease (AD). However, there
Michael B McFerrin et al.
Annals of clinical and translational neurology, 4(7), 466-477 (2017-07-12)
The highly conserved 14-3-3 proteins interact with key players involved in Parkinson's disease (PD) and other neurodegenerative disorders. We recently demonstrated that 14-3-3 phosphorylation is increased in PD models and that increased 14-3-3 phosphorylation reduces the neuroprotective effects of 14-3-3
Cheril Tapia-Rojas et al.
Journal of neurochemistry, 144(4), 443-465 (2017-12-15)
Alzheimer's disease (AD) is a neurodegenerative pathology characterized by aggregates of amyloid-β (Aβ) and phosphorylated tau protein, synaptic dysfunction, and spatial memory impairment. The Wnt signaling pathway has several key functions in the adult brain and has been associated with

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