biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CTSD(1509)
General description
兔抗组织蛋白酶D(Ab-2)多克隆抗体识别乳腺癌组织中组织蛋白酶D的~52 kDa潜态(latent)形式和 ~34 kDa亚基。它经过验证可用于冷冻和石蜡切片的WB和IHC分析。
可识别乳腺癌组织提取物中组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的亚基，但不可识别〜14 kDa亚基。
纯化的兔多克隆抗体。可识别组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的（但不包括〜14 kDa）活性亚基。
Immunogen
人
纯化的人肝组织蛋白酶D
Application
冷冻切片（5 µg/ml）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
Packaging
请参考特定浓度批号的标签。
Physical form
溶于50 mM磷酸钠缓冲液（pH 7.5），含0.2％明胶。
Analysis Note
阳性对照
HT1080浓缩条件培养基或乳腺癌组织
HT1080浓缩条件培养基或乳腺癌组织
Other Notes
Rochefort, H. 1992.Eur. J. Cancer28A, 1780.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
为了获得最佳的免疫印迹结果，应在上样前将HT1080细胞的条件培养基浓缩10-20X。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10-13 - German Storage Class 10 to 13
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