生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... CTSD(1509)
一般描述
兔抗组织蛋白酶D(Ab-2)多克隆抗体识别乳腺癌组织中组织蛋白酶D的~52 kDa潜态(latent)形式和 ~34 kDa亚基。它经过验证可用于冷冻和石蜡切片的WB和IHC分析。
可识别乳腺癌组织提取物中组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的亚基，但不可识别〜14 kDa亚基。
纯化的兔多克隆抗体。可识别组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的（但不包括〜14 kDa）活性亚基。
免疫原
人
纯化的人肝组织蛋白酶D
应用
冷冻切片（5 µg/ml）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
包装
请参考特定浓度批号的标签。
外形
溶于50 mM磷酸钠缓冲液（pH 7.5），含0.2％明胶。
分析说明
阳性对照
HT1080浓缩条件培养基或乳腺癌组织
HT1080浓缩条件培养基或乳腺癌组织
其他说明
Rochefort, H. 1992.Eur. J. Cancer28A, 1780.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
为了获得最佳的免疫印迹结果，应在上样前将HT1080细胞的条件培养基浓缩10-20X。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：标准处理（A）
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储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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