MAB1330
Anti-Collagen Type II (COL2A1) Antibody
CHEMICON®, mouse monoclonal, COLL-II
别名:
Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
COLL-II, monoclonal
Species reactivity:
sheep, human, bovine, rat, pig, mouse, canine
Application:
ELISA, IHC
Citations:
44
产品名称
Anti-Collagen Type II Antibody, clone COLL-II, ascites fluid, clone COLL-II, Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
COLL-II, monoclonal
species reactivity
sheep, human, bovine, rat, pig, mouse, canine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... COL2A1(1280)
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Immunogen
Human (cartilage specific) CNBr-cleaved collagen II.
Application
Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.
Western Blot: 1:1000-1:2000 with chemiluminescent detection
ELISA at 1:100-1:500, pepsin treated samples.
Optimal working dilutions must be determined by the end user.
Western Blot: 1:1000-1:2000 with chemiluminescent detection
ELISA at 1:100-1:500, pepsin treated samples.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.
Biochem/physiol Actions
Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.
Physical form
Ascites fluid containing no preservatives.
Unpurified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Cartilage in lung or fetus
Cartilage in lung or fetus
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Sarvpreet Singh et al.
Journal of cellular and molecular medicine, 16(4), 877-887 (2011-07-19)
Irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (MSC), which play a pivotal role in bone regeneration. As a consequence of radiotherapy, bones may develop osteoradionecrosis. When irradiating human bone-derived MSC in vitro with increasing
Danijela Menicanin et al.
Stem cells and development, 23(9), 1001-1011 (2013-12-20)
Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived after 8
Aruna Prasopthum et al.
ACS applied materials & interfaces, 11(21), 18896-18906 (2019-05-09)
The effect of topography in three-dimensional (3D) printed polymer scaffolds on stem cell differentiation is a significantly underexplored area. Compared to two-dimensional (2D) biomaterials on which various well-defined topographies have been incorporated and shown to direct a range of cell behaviors including adhesion
Yang-Jun Lao et al.
Orthopaedic surgery, 10(1), 56-63 (2018-02-13)
To investigate the effect of accumulated spinal axial biomechanical loading on mice lumbar disc and the feasibility of applying this method to establish a mice intervertebral disc degeneration model using a custom-made hot plate cage. In previous studies, we observed
Xin Lu et al.
International journal of molecular medicine, 37(2), 509-516 (2015-12-29)
The aim of the present study was to examine the expression levels and role of hsa-miR-15a in osteoarthritis (OA), as well as the associated mechanisms. The expression levels of hsa-miR-15a and A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type
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