biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
CAT-1, monoclonal
species reactivity
E. coli, vertebrates
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
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Immunogen
Chloramphenicol acetyl transferase
Application
Detect Chloramphenicol Acetyltransferase using this Anti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1 validated for use in WB & IC.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western blot: 1:2000-1:5000+
Immunocytochemistry: 4% PFA fixed cells (5-10′ RT, block with 3% normal secondary serum in PBS with 0.1% triton X-100 to permeabilize cell membrane. Primary antibody, 1:400, 1 hour.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 4% PFA fixed cells (5-10′ RT, block with 3% normal secondary serum in PBS with 0.1% triton X-100 to permeabilize cell membrane. Primary antibody, 1:400, 1 hour.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Chloramphenicol acetyl transferase (CAT). CAT is used as a tag in many systems and is also used in transcription assays.
Physical form
Ascites fluid. Liquid. Contains no preservative.
Preparation Note
Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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