Anti-ACAT1 (CES1), clone 10E8

Anti-ACAT1 (CES1), clone 10E8, Cat. No. MABC1101, is a mouse monoclona antibody that detects Liver carboxylesterase 1 and has been tested for use in ELISA and Western Blotting.

ELISA Western Blotting

ELISA Analysis: A representative lot detected ACAT1 (CES1) in ELISA applications (Na, K., et. al. (2013). Int J Cancer. 133(2):408-15).

Western Blotting Analysis: A representative lot detected ACAT1 (CES1) in Western Blotting applications (Na, K., et. al. (2013). Int J Cancer. 133(2):408-15).

Clone 10E8 is a mouse monoclonal antibody that specifically detects human liver carboxyesterase 1 (ACAT1). It targets an epitope with in 14 amino acids from the C-terminal region. It is expected to react with all isoforms of ACAT1.

A linear peptide corresponding to 14 amino acids from the C-terminal region of human Liver Carboxyesterase 1 (ACAT1).

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in human liver tissue lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected ACAT1 (CES1) in human liver tissue lysates.

Liver carboxylesterase 1 (UniProt: P23141; also known as EC: 3.1.1.13, Acyl-coenzyme A:cholesterol acyltransferase, ACAT, Brain carboxylesterase hBr1, Carboxylesterase 1, CE-1, hCE-1, Cocaine carboxylesterase, Egasyn, HMSE, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, REH, Serine esterase 1, Triacylglycerol hydrolase, TGH) is encoded by the CES1 (also known as CES2, SES1) gene (Gene ID: 1066) in human. ACAT1 is a members of the serine hydrolase superfamily that is localized within the lumen of the endoplasmic reticulum in many tissues, but is predominantly present in the liver. It catalyzes the ester cleavage of a large number of structurally diverse ester- or amide-containing substrates into the corresponding alcohol and carboxylic acid. It is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It also possesses triglyceride and cholesterol ester hydrolase activity and may play an important role in regulating lipid metabolism. It is highly expressed in the liver, but can be found to a lesser extent in the intestine, macrophages, and other tissues. ACAT1 can exist either in a homotrimeric or a homohexameric form. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce the mature enzyme. This cleavage can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. Three isoforms of ACAT have been described that are produced by alternative splicing. (Ref.: Laizure., SC et al., (2013). Pharmacotherapy. 33(2): 210-222; Na, K., et al (2013). Int. J. Cancer: 133, 408-416).

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