Anti-BRMS1, clone 3a1.21

Anti-BRMS1, clone 3a1.21, Cat. No. MABC1753, is a mouse monoclonal antibody that detects Breast cancer metastasis-suppressor 1 and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Immunoprecipitation Western Blotting

Immunohistochemistry Analysis: A representative lot detected BRMS1 in Immunohistochemistry applications (Slipicevic, A., et. al. (2012). BMC Cancer. 12:73; Zhang, G., et. al. (2012). PLoS One. 7(9):e45037).

Immunoprecipitation Analysis: A representative lot immunoprecipitated BRMS1 in Immunoprecipitation applications (Hurst, D.R., et. al. (2006). Biochem Biophys Res Commun. 348(4):1429-35).

Western Blotting Analysis: A representative lot detected BRMS1 in Western Blotting applications (Hurst, D.R., et. al. (2006). Biochem Biophys Res Commun. 348(4):1429-35).

Clone 3a1.21 is a mouse monoclonal antibody that detects human Breast cancer metastasis-suppressor 1. It targets an epitope within the first 50 amino acids from the N-terminal region.

GST-tagged full-length recombinant human Breast cancer metastasis-suppressor 1.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Breast cancer metastasis-suppressor 1 (UniProt: Q9HCU9, also known as BRMS1) is encoded by the BRMS1 gene (Gene ID: 25855) in human. BRMS1 is an homohexameric, predominantly nuclear protein with two nuclear localization sequences (aa 198-205 and 238-244) but may also have a cytoplasmic role. Its cytoplasmic expression has been linked to higher disease-free survival in cases of malignant melanoma. It is a member of the mSin3-HDAC transcription co-repressor complex. It is reported to suppress metastasis in multiple human and murine carcinoma cell lines by altering the expression of both coding and non-coding metastasis associated genes. It down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RelA at lysine 310 and HDAC1 binding to promoter regions. It is also shown to down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. HSP90 is reported to stabilize the levels of BRMS1 in cells, which may be important in maintaining the functional role of BRMS1 as a metastasis suppression. (Ref.: Hurst, DR et al., PLoS One. 2013;8(2); e55966; Hurst, DR et al. (2006). Biochem. Biophys. Res. Commun. 348(4); 1429- 1435; Slipicevic, A., et al. (2012). BMC Cancer 12; 73).

Evaluated by Western Blotting in HepG2 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BRMS1 in HepG2 cell lysates.