Anti-RUNX1 Antibody, clone 7H12.1

Runt-related transcription factor 1 (UniProt Q01196; also known as Acute myeloid leukemia 1 protein, CBF-alpha-2, Core-binding factor subunit alpha-2, Oncogene AML-1, PEA2-alpha B, PEBP2-alpha B, Polyomavirus enhancer-binding protein 2 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit) is encoded by the RUNX1 (also known as AML1, CBFA2) gene (Gene ID 861) in human. RUNX1 is a runt-related transcription factor family member that plays a role in the differentiation of hematopoietic stem cells into mature blood cells. RUNX1 forms a core-binding factor (CBF) complex with core binding factor β (CBFβ; encoded by the CBFB gene), and both RUNX1 and CBFB genes are well known targets of chromosomal translocations in acute myeloid leukemia (AML), generating the leukemogenic fusion proteins AML1-ETO and CBFB-MYH11. RUNX1 contains an N-terminal Runt homology domain (RHD; a.a. 50-178) that mediates protein-protein interaction, followed by a DNA-binding region (a.a. 80-177) that mediates the binding of the core consensus sequence TGTGGNNN (where NNN can represent either TTT or TCA). RUNX1 can bind DNA as a monomer, but its DNA-binding affinity is enhanced by 10-fold when complexed with CBFβ.

~52-60 kDa observed. Target band(s) size can vary depending on phosphorylation and isoform(s) present in sample(s). 50.70 kDa (soform AML-1A), 48.74 kDa (AML-1B), 27.43 kDa (AML-1C), 28.23 kDa (AML-1E), 51.82 kDa (AML-1G), 49.08 kDa (AML-1H), 50.17 kDa (AML-1I), 37.73 kDa (AML-1L; AML1-delta N) calculated. Uncharacterized band(s) may appear in some lysates.

Epitope: Region between DNA-binding domain and KAT6A-binding domain.

GST-tagged recombinant fragment from the region between the DNA-binding domain and the KAT6A-binding domain of human RUNX1.

Anti-RUNX1 Antibody, clone 7H12.1 is an antibody against RUNX1 for use in Western Blotting.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Western Blotting Analysis: A 1:500 dilution from a representative lot detected RUNX1 in 10 µg of human TF-1 erythroleukemic cell and murine RAW264.7 macrophage lysates.

Expected to react with isoform AML-1A, AML-1B, AML-1E, AML-1G, AML-1H, AML-1I, AML-1L (AML1-delta N), but not AML-1FA, AML-1FB, or AML-1FC. Reactivity toward isoform AML-1C is likely, but has not been confirmed.

Unpurified

Mouse monoclonal IgG1κ hybridoma culture supernatant without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting Analysis in K562 cell lysate:

Western Blotting Analysis: A 1:500 dilution of this antibody detected RUNX1 in 10 µg of human K562 erythroleukemic cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.