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Merck
CN

MABF187

Sigma-Aldrich

Anti-KARS Antibody, clone 8G12C1

ascites fluid, clone 8G12C1, from mouse

别名:

Lysine-tRNA ligase, Lysyl-tRNA synthetase, LysRS

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

mouse

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

8G12C1, monoclonal

种属反应性

human

技术

immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG1

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... KARS1(3735)

一般描述

KARS, also known as Lysyl-tRNA synthetase (LysRS), is the enzyme that connects the lysine to its specific tRNA for use in protein translation. KARS is also a powerful inflammatory agent when released into extracellular matrix as one might expect. KARS is largely cytoplasmically localized or localized to internal membranes but isoforms are found in the mitochondria and nucleus as well. KARS became the focus of much attention with HIV-1 as KARS interacted with HIV-1’s GAG protein and facilitated the selective packaging of tRNA3(lys) nucleic acid which acted as the primer for viral reverse transcription and initiation of viral gene expression. There is also at least one genetic disease linked to KARS. It is the cause of Charcot-Marie-Tooth neuropathy a progressive peripheral nervous system disease characterized by progressive weakness and atrophy of the muscles and demyelination and deterioration the peripheral nerves and axons. EMD-Millipore’s Anti-KARS mouse monoclonal antibody has been tested in western blot on recombinant protein and in paraffin embedded immunohistochemistry on human cervical carcinoma tissue sections.
~24, ~44, and ~50 kDa observed. This antibody recognizes truncated MBP-KARS at ~24 kDa, full length KARS at ~44 kDa, and transfected CHO-K1 cell lysate at ~50 kDa

免疫原

Purified recombinant fragment of human KARS expressed in E. Coli.

应用

Anti-KARS Antibody, clone 8G12C1 is a highly specific mouse monoclonal antibody, that targets Lysyl-tRNA synthetase & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KARS in human cervical carcinoma and human tonsil tissues.

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

制备说明

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates
Evaluated by Western Blotting in truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected KARS in truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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