Anti-Ly49A Antibody, clone A1

30.50 kDa calculated.

T-cell surface glycoprotein YE1/48 (UniProt: P20937; also known as Lymphocyte antigen 49a, Ly-49a, T lymphocyte antigen A1) is encoded by the Klra1 (also known as Ly-49, Ly-49a, Ly49, Ly49A) gene in murine species. LY49A is a single-pass type II membrane protein that serves as an inhibitory receptor on natural killer (NK) cells for H-2d(Dd) MHC class I molecules on target cells. It inhibits the activity of NK cells thereby preventing cell lysis. Ly49A-mediated NK cell inhibition is shown to be dependent on a lateral binding site, which is located beneath the peptide-binding platform of Dd. Ly49A is reported to contribute to target cell adhesion and accumulate at synapses with Dd-expressing target cells when NK cells themselves lack Dd. In cases where NK cells display Dd expression Ly49A does not contribute to adhesion. Higher expression of LY49A is observed in T-lymphoma cell lines and normal lymphocytes exhibit low expression. LY49A has a cytoplasmic domain (aa 1-44), a transmembrane domain (aa 45-66), and an extracellular domain (aa 67-262). (Ref.: Back, J., et al. (2007). Proc. Natl. Acad. Sci. USA 104(10); 3978 3983).

EL-4 T lymphoma cells.

Immunoprecipitation Analysis: A representative lot detected Ly49A in Immunoprecipitation applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86).

Immunocytochemistry Analysis: A representative lot detected Ly49A in Immunocytochemistry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83).

Flow Cytometry Analysis: A representative lot detected Ly49A in Flow Cytometry applications (Nagasawa, R., et. al. (1987). J Immunol. 138(3):815-24; Yokoyama, W.M., et. al. (1989). J Immunol. 143(4):1379-86; Back, J., et. al. (2007). Proc Natl Acad Sci U S A. 104(10):3978-83; Silver, E.T., et. al. (2000). J Immunol. 165(4):1771-81).

This mouse monoclonal Anti-Ly49A, clone A1, Cat. No. MABF2090 is tested for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation, for the detection of Lymphocyte antigen 49a.

Clone A1 specifically detects Lymphocyte antigen 49a (LY49A) in murine species.

Format: Purified

Evaluated by Flow Cytometry in EL-4 T lymphoma cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Ly49A in 1X10E6 EL-4 T lymphoma cells.

Concentration: Please refer to lot specific datasheet.