Anti-Dengue Virus 1, clone 271

Anti-Dengue Virus 1 NS1, clone 271, Cat. No. MABF2188. is a mouse monoclonal antibody that detects DENV1 NS1 protein and has been tested for use in ELISA and Flow Cytometry.

ELISA Flow Cytometry

ELISA Analysis: A representative lot detected Dengue Virus 1 NS1 protein in ELISA applications (Bosch, I., et. al. (2017). Sci. Transl. Med. 9(409); eaan1589).

Flow Cytometry Analysis: A representative lot detected Dengue Virus 1 NS1 protein in Flow Cytometry applications (Bosch, I., et. al. (2017). Sci. Transl. Med. 9(409); eaan1589).

Clone 271 is a mouse monoclonal antibody that detects Dengue virus NS1 protein. Also, displays reactivity with Dengue virus 3 NS1 protein. However, when paired with Dengue virus 1 NS 1 specific antibody (Clone 912), such combination only recognizes Dengue virus 1 NS1 protein and not Dengue virus 3 NS1 protein. It targets an epitope within 16 amino acids from the wing domain.

Purified recombinant Dengue virus 1 NS1 protein expressed in mammalian cells.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. (Ref.: Chen, RU et al. (2018). J Biomed Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).

Evaluated by ELISA with DENV1 NS1 recombinant protein.

ELISA Analysis: 5 µg/mL of this antibody detected DENV1 NS1 recombinant protein.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.