Anti-iSEC1, clone GT42

Anti-iSEC1, clone GT42, Cat. No. MABF2226, is a rat monoclonal anntibody that detects intestinal secretory cell protein 1 (iSEC1) and has been tested for use in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Immunocytochemistry

Flow Cytometry Analysis: A representative lot detected iSEC1 in Flow Cytometry applications (Kojima, T., et. al. (2016). Sci Rep. ;6:36457).

Immunocytochemistry Analysis: A representative lot detected iSEC1 in Immunocytochemistry applications (Kojima, T., et. al. (2016). Sci Rep. ;6:36457).

GT42 is a rat monoclonal antibody that specifically detects murine ISEC1 (intestinal secretory cell protein 1), but not the iSEC2.

iSEC1-expressing NIH/3T3 cells.

Stable for 1 year at 2-8°C from date of receipt.

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Flow Cytometry in iSEC1-expressing NIH3T3 cells

Flow Cytometry Analysis: 1 µg of this antibody detected iSEC1 in iSEC1-expressing NIH3T3 cells.

ISEC1 protein, intestinal secretory cell protein 1 (UniProt: A0A077K8V5) is encoded by the Gm609 (also known as isec1) gene (Gene ID: 208166) in murine species. iSEC1 is a D200 homologue that is expressed mainly in the small intestine, but some expression is observed in the stomach, duodenum, jejunum, ileum, cecum, and colon. It is not detected in esophagus or other tissues. It can bind to CD200R, but not to other members of the CD200R family. Binding of iSEC1 to CD200R on intraepithelial lymphocytes (IELs) results in the suppression of cytokine production and cytolytic activity activated by IELs. Hence, iSEC1 is considered as a negative regulator of IELs function. iSEC1 is synthesized with a signal peptide (aa 1-23) that is cleaved off in the mature form. The mature form exists as a disulfide-linked homodimer. (Ref.: Kojima, T., et al. (2016). Sci. Rep. 6: 36457).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.