Anti-BLAST-2 (CD23), clone EBVCS2

Anti-BLAST-2 (CD23), clone EBVCS2, Cat. No. MABF2271, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tested for use in Flow Cytometry and Immunoprecipitation.

Flow Cytometry Immunoprecipitation

Immunoprecipitation Analysis: A representative lot immunoprecipitated BLAST-2 (CD23) in Immunoprecipitation applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).

Flow Cytometry Analysis: A representative lot detected BLAST-2 (CD23) in Flow Cytometry applications (Balan, S., et. al. (2014). J Immunol. 193(4):1622-35).

Clone EBVCS2 is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (CD23; BLAST-2).

EBV-transformed human cells.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Flow Cytometry in Raji cells.

Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in one million Raji cells.

Low affinity immunoglobulin epsilon Fc receptor (UniProt: P06734; also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23) is encoded by the FCER2 (also known as CD23A, CLEC4J, FCE2, IGEBF) gene (Gene ID: 2208) in human. Epstein-Barr virus (EBV) is reported to transform human B lymphocytes and is a causative agent for Burkitt s lymphoma and infectious mononucleosis. EBV induces the expression of one or more antigens (e.g., CD23) on the surface of adult human B-lymphocytes soon after infection. CD23 is a homotrimeric, single-pass type II membrane protein that serves as a low-affinity receptor for Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in the differentiation of B-cells. CD23 can also exist as a soluble excreted form, sCD23 (aa 150-321), which is produced by ADAM10-mediated ectoderm shedding. CD23 has a cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-47), and an extracellular domain (aa 48-321). Clone EBVCS2 can specifically detect antigens that are present on the surface of EBV-transformed cells. These antigens are also recognized by autochthonous cytotoxic T cells specific for EBV-transformed cells. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460).

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