Anti-CLM-4 (CD300c), clone TX52

Anti-CLM-4 (CD300c), clone TX52, Cat. No. MABF2328 is a rat monoclonal antibody that detects CMRF-35-like molecule 4 (CD300c) and has been tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.

Flow Cytometry Immunoprecipitation Western Blotting

Immunoprecipitation Analysis: A representative lot immunoprecipitated CLM-4 (CD300c) in Immunoprecipitation applications (Nakahashi, C., et. al. (2007). J Immunol. 178(2):765-70).

Western Blotting Analysis: A representative lot detected CLM-4 (CD300c) in Western Blotting applications (Nakahashi, C., et. al. (2007). J Immunol. 178(2):765-70).

Flow Cytometry Analysis: A representative lot detected CLM-4 (CD300c) in Flow Cytometry applications (Totsuka, N., et. al. (2014). Nat Commun. 5:4710).

Clone TX52 is a rat monoclonal antibody that specifically detects murine CMRF-35-like molecule 4 (CD300c).

Ba/F3 cells transfected with mouse CMRF-35-like molecule 4 (CD300c).

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Purified rat monoclonal antibody IgG2b in PBS without azide.

CMRF-35-like molecule 4(UniProt: Q7TSN2; also known as CLM-4, CD300C molecule 2, Dendritic cell-derived Ig-like receptor 1, DIgR1, Immunoglobulin superfamily member 7, IgSF7, Leukocyte mono-Ig-like receptor 2, Myeloid-associated immunoglobulin-like receptor 2, MAIR-2, MAIR-II) is encoded by the Cd300c2 (also known as Cd300c, Clm4, Igsf7, Lmir2) gene (Gene ID: 140497) in murine species. CLM-4 is a single-pass type I membrane protein that is present on the surface of mast cells, dendritic cells, peritoneal macrophages and a subset of B-cells. It acts as an activating receptor in mast cells and macrophages. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-187), a transmembrane domain (aa 188-208), and a cytoplasmic domain (aa 209-228). CLM-4 is shown to associate with DAP12, which mediates activating signals, resulting in inflammatory cytokine secretion from macrophages. Cross-linking CLM-4 with this monoclonal antibody is reported to induced secretion of a significant amount of the inflammatory cytokines TNF-alpha and IL-6 from DAP12 -/- as well as wild-type (WT) peritoneal macrophages. Mice deficient in CLM-4 are shown to be more susceptible to caecal ligation and puncture (CLP)-induced peritonitis. (Ref.: Totsuka, N., et al. (2014). Nat. Commun. 5; 4710; Nakahashi, C. et al. (2007). J. Immunol. 178(2); 765-770).

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CLM-4 (CD300c) in mouse spleen tissue lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.