Anti-Extrahepatic biliary cells Antibody, clone hGB10

The gallbladder is a small, distensible pouch-like organ that concentrates and stores bile produced by the liver that helps break down dietary fats. Bile flows out of the liver via hepatic ducts that join with the cystic duct, which conveys bile to and from the gall bladder. Gallbladder is derived from the foregut endoderm during embryonic development. In an un-distended state, its mucosa appears to be thrown into many folds. Its lumen is lined with a high columnar epithelium. This epithelial layer rests on a highly vascularized lamina propria. The biliary epithelium shows a morphological heterogeneity that is strictly associated with a variety of functions performed at the different levels of the biliary tree. These epithelial cells are also involved in absorption of water and inorganic salts and help to concentrate bile. The connective tissue wall of gallbladder contains abundant elastic fibers and layers of smooth muscles that run obliquely. Between the smooth muscle layers and serosa lies a thick layer subserosal layer of connective tissue. The availability of antibodies that preferentially mark cell populations within the extrahepatic biliary system are useful for identification and classification of gallbladder cells in relation to bile physiology, gallstone formation, cell plasticity and renewal, metaplasia, and tumor formation. Clone hBG10 is reported to specifically detect a subset of gallbladder mucosal epithelium with apical bias and peribiliary glands. (Ref.: Galivo, FH., et al. (2015). Stem Cell Res. 15(1); 172-181).

Extrahepatic human biliary cells

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human gallbladder tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected a subset of mucosal epithelium with apical bias and peribiliary glands in human gallbladder tissue sections.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Extrahepatic biliary cells in Immunofluorescence applications (Galivo, F.H., et al. (2015). Stem Cell Res. 15(1):172-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Extrahepatic biliary cells, clone hGB10, Cat. No. MABS2190, is a mouse monoclonal antibody that detects subset of gallbladder mucosal epitheliums with apical bias and is tested in Immunofluorescence and Immunohistochemistry (Paraffin).

Clone hGB10 is a mouse monoclonal antibody that detects a subset of mucosal epithelium with apical bias in human gallbladder.

Purified mouse monoclonal antibody IgG1 in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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