生物来源
mouse
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
AR38.2, monoclonal
种属反应性
human, mouse
包装
antibody small pack of 25 μL
技术
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
靶向翻译后修饰
unmodified
基因信息
human ... CDH1(999)
一般描述
Fizzy-related protein homolog (UniProt: Q9R1K5; also known as Fzr, Cdh1/Hct1 homolog) is encoded by the Fzr1 (also known as Fyr, Fzr) gene (Gene ID: 56371) in murine species. Cdh1 is a substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex that associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition Cdh1 is phosphorylated, leading to its dissociation from the APC/C. It has been shown that Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while CDC14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to ubiquitination and degradation of Polo-like kinase 1 (Plk1) and prevents entry into mitosis. Following DNA repair, Cdk1 and Plk1 are reactivated and cell cycle progresses from G2 into mitosis phase.
~54 kDa observed; 54.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
免疫原
His-tagged full-length recombinant mouse Fizzy-related protein homolog (Cdh1).
应用
Anti-Cdh1, clone AR38, Cat. No. MABT1323, is a mouse monoclonal antibody that detects Cdh1/FZR1 and has been tested for use in Western Blotting.
Western Blotting Analysis: A representative lot detected Cdh1 in Western Blotting applications (Garzon, J., et. al. (2017). Oncogene. 36(42):5808-5818).
生化/生理作用
Clone AR38.2 detects Cdh1 (Fizzy-related protein homolog) in human and mouse cells.
外形
Format: Purified
分析说明
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdh1 in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdh1 in HeLa cell lysate.
其他说明
Concentration: Please refer to lot specific datasheet.
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法规信息
新产品
Sahezeel Awadia et al.
The Journal of biological chemistry, 299(6), 104786-104786 (2023-05-06)
The E3 ubiquitin ligase APC/C-Cdh1 maintains the G0/G1 state, and its inactivation is required for cell cycle entry. We reveal a novel role for Fas-associated protein with death domain (FADD) in the cell cycle through its function as an inhibitor
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