biological source
mouse
antibody product type
primary antibodies
clone
14, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~87 kDa MLH1 protein.
Recognizes the ~83 kDa MLH1 protein in LoVo cells.
This Anti-MLH1 (Ab-1) Mouse mAb (14) is validated for use in Immunoblotting, Paraffin Sections for the detection of MLH1 (Ab-1).
Immunogen
Human
recombinant, human MLH1
Application
Immunoblotting (1 µg/ml)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
HCT116 cells
HCT116 cells
Positive Control
LoVo cells or colon tissue
LoVo cells or colon tissue
Other Notes
For paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
Kane, M.F., et al. 1997. Cancer Res.57, 808.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
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