生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
14, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~87 kDa MLH1 protein.
Recognizes the ~83 kDa MLH1 protein in LoVo cells.
This Anti-MLH1 (Ab-1) Mouse mAb (14) is validated for use in Immunoblotting, Paraffin Sections for the detection of MLH1 (Ab-1).
免疫原
Human
recombinant, human MLH1
应用
Immunoblotting (1 µg/ml)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
外形
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
分析说明
Negative Control
HCT116 cells
HCT116 cells
Positive Control
LoVo cells or colon tissue
LoVo cells or colon tissue
其他说明
For paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
Kane, M.F., et al. 1997. Cancer Res.57, 808.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
新产品
此项目有
T Fujiwara et al.
The American journal of pathology, 153(4), 1063-1078 (1998-10-20)
A subset of hereditary and sporadic colorectal carcinomas is defined by microsatellite instability (MSI), but the spectra of gene mutations have not been characterized extensively. Thirty-nine hereditary nonpolyposis colorectal cancer syndrome carcinomas (HNPCCa) and 57 sporadic right-sided colonic carcinomas (SRSCCa)
Christophe Papin et al.
Journal of experimental & clinical cancer research : CR, 42(1), 301-301 (2023-11-14)
Inherited defects in the base-excision repair gene MBD4 predispose individuals to adenomatous polyposis and colorectal cancer, which is characterized by an accumulation of C > T transitions resulting from spontaneous deamination of 5'-methylcytosine. Here, we have investigated the potential role of MBD4
Samar Hassen et al.
Journal of experimental & clinical cancer research : CR, 30, 100-100 (2011-10-25)
A broad population-based assay to detect individuals with Lynch Syndrome (LS) before they develop cancer would save lives and healthcare dollars via cancer prevention. LS is caused by a germline mutation in a DNA mismatch repair (MMR) gene, especially protein
Gregoriy A Dokshin et al.
Developmental cell, 52(1), 53-68 (2019-12-17)
GCNA proteins are expressed across eukarya in pluripotent cells and have conserved functions in fertility. GCNA homologs Spartan (DVC-1) and Wss1 resolve DNA-protein crosslinks (DPCs), including Topoisomerase-DNA adducts, during DNA replication. Here, we show that GCNA mutants in mouse and
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持