生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
porcine, human, rat, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence))
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... UCHL1(7345)
一般描述
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.
Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.
This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).
免疫原
a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
应用
Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)
外形
Undiluted serum.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Mouse dorsal root nerve fibers
Mouse dorsal root nerve fibers
其他说明
Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
PGP9.5 is also overexpressed in some cancers.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
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