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Merck
CN

OP20

Anti-c-Abl (Ab-3) Mouse mAb (24-21)

liquid, clone 24-21, Calbiochem®

别名:

Anti-p150, Anti-Abl

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NACRES:
NA.41
UNSPSC Code:
12352203

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产品名称

Anti-c-Abl (Ab-3) Mouse mAb (24-21), liquid, clone 24-21, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

24-21, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (1-5 µg/mL)
Immunofluorescence
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (not recommended))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ABL1(25)

Analysis Note

Positive Control
K562 (human bcr/abl), HL-60 (normal abl), and ANN-1 (murine abl) cells

Application

Immunoblotting (1-5 µg/ml)

Immunofluorescence (see application references)

Immunoprecipitation (1-2 µg/sample)

Neutralization Studies (not recommended)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins.
Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity.
  • Antibody Target Gene Symbol: ABL1
  • Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl
  • Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase
  • Hu Entrez ID: 25 (Related Antibodies: PK1006PK1013OP19)
  • Mu Entrez ID: 11350
  • Rat Entrez ID: 311860

    • Immunogen

    a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE

    Other Notes

    Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types.
    Wiedemann, L.M., et al. 1988. Blood71, 349.
    Stam, K., et al. 1985. N. Engl. J. Med.313, 1429.
    Konopka, J.B., et al. 1984. Cell37, 1035.
    Prywes, R., et al. 1983. Cell34, 569.
    de Klein, A., et al. 1982. Nature300, 765.
    Reynolds, F.H., Jr., et al. 1980. J. Virol.36, 374.
    Reynolds, F.H., Jr., et al. 1978. Proc. Natl. Acad. Sci. USA75, 3974.
    Witte, O.N., et al. 1978. Proc. Natl. Acad. Sci. USA75, 2488.
    Abelson, H.T. and Rabstein, L.S. 1970. Cancer Res.30, 2213.

    Packaging

    Please refer to vial label for lot-specific concentration.

    Physical form

    In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    存储类别

    10 - Combustible liquids

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

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    Alexander J M Blakes et al.
    European journal of human genetics : EJHG, 29(4), 593-603 (2020-11-24)
    ABL1 is a proto-oncogene encoding a nonreceptor tyrosine kinase, best known in the somatic BCR-ABL fusion gene associated with chronic myeloid leukaemia. Recently, germline missense variants in ABL1 have been found to cause an autosomal dominant developmental syndrome with congenital
    Cong Fang et al.
    Cancer research, 70(21), 8299-8308 (2010-09-15)
    Oncogenic kinase activity and the resulting aberrant growth and survival signaling are a common driving force of cancer. Accordingly, many successful molecularly targeted anticancer therapeutics are directed at inhibiting kinase activity. To assess kinase activity in minute patient samples, we
    Shigeru Matsumura et al.
    Nature communications, 7, ncomms11858-ncomms11858 (2016-06-14)
    Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of the cell division axis, the molecular mechanisms that translate interphase adhesion geometry to the mitotic spindle orientation remain elusive. Here, we show that the cellular
    Tamjeed Saleh et al.
    Nature structural & molecular biology, 24(11), 893-901 (2017-09-26)
    The activity of protein kinases is often regulated in an intramolecular fashion by signaling domains, which feature several phosphorylation or protein-docking sites. How kinases integrate such distinct binding and signaling events to regulate their activities is unclear, especially in quantitative
    P Ballerini et al.
    Leukemia, 26(11), 2384-2389 (2012-04-20)
    Myeloproliferative neoplasms are frequently associated with aberrant constitutive tyrosine kinase (TK) activity resulting from chimaeric fusion genes or point mutations such as BCR-ABL1 or JAK2 V617F. We report here the cloning and functional characterization of two novel fusion genes BCR-RET
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