生物来源
mouse
抗体产品类型
primary antibodies
克隆
22, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
mouse, human, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
一般描述
This product has been discontinued.
Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.
Purified mouse monoclonal antibody, generated by immunizing BALB/c x C57B1/6 F1 mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~21 kDa p21WAF1 protein.
免疫原
Epitope: within amino acids 20-40 of human p21WAF1
recombinant, mouse p21WAF1
应用
Frozen Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
包装
Please refer to vial label for lot-specific concentration.
分析说明
Positive Control
Induced rat or mouse embryo fibroblasts
Induced rat or mouse embryo fibroblasts
其他说明
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence detection methods. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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