biological source
mouse
antibody product type
primary antibodies
clone
22, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
General description
This product has been discontinued.
Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.
Purified mouse monoclonal antibody, generated by immunizing BALB/c x C57B1/6 F1 mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~21 kDa p21WAF1 protein.
Immunogen
Epitope: within amino acids 20-40 of human p21WAF1
recombinant, mouse p21WAF1
Application
Frozen Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
Immunoblotting (3 µg/ml)
Immunoprecipitation (1 µg/ml)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Induced rat or mouse embryo fibroblasts
Induced rat or mouse embryo fibroblasts
Other Notes
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence detection methods. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
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