生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG
运输
wet ice
储存温度
2-8°C
一般描述
Anti-GluR1, rabbit polyclonal, recognizes the ~105-107 kDa GluR1 in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain. It is validated for WB and IF.
Purified rabbit polyclonal antibody. Recognizes the ~105-107 kDa GluR1 protein.
Recognizes the ~105-107 kDa GluR1 protein in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain extracts.
免疫原
a synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
应用
Immunoblotting 1-5 µg/ml)
Immunofluorescence (10-20 µg/ml, see application references)
Immunofluorescence (10-20 µg/ml, see application references)
包装
Please refer to vial label for lot-specific concentration.
分析说明
Positive Control
D425 Med cells, hippocampal neurons
D425 Med cells, hippocampal neurons
其他说明
Also recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
Zuo, J. et al. 1997. Nature388, 769.
McHugh, T.J., et al. 1996. Cell87, 1339.
Tsien, J.Z., et al. 1996. Cell87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci.17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature361, 31.
Lomeli, H., et al. 1993. FEBS Lett.315, 318.
Molnar, E. et al. 1993. Neuroscience53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev.16, 13.
Beal, M.F. 1992. FASEB J.6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci.648, 132.
McHugh, T.J., et al. 1996. Cell87, 1339.
Tsien, J.Z., et al. 1996. Cell87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci.17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature361, 31.
Lomeli, H., et al. 1993. FEBS Lett.315, 318.
Molnar, E. et al. 1993. Neuroscience53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev.16, 13.
Beal, M.F. 1992. FASEB J.6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci.648, 132.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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