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Merck
CN

PC317L

Sigma-Aldrich

Anti-Angiogenin Goat pAb

lyophilized, Calbiochem®

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UNSPSC代码:
12352203

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生物来源

goat

质量水平

100

抗体产品类型

primary antibodies

克隆

polyclonal

表单

lyophilized

不包含

preservative

种属反应性

human

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG

运输

ambient

储存温度

−20°C

一般描述

Anti-Angiogenin, goat polyclonal, recognizes angiogenin and can neutralize the activity of human recombinant angiogenin. It is validated for ELISA, Western blotting, and neutralization studies.
Immunoaffinity purified goat polyclonal antibody. Recognizes the ~14 kDa angiogenin protein.
Recognizes angiogenin.
  • Antibody Target Gene Symbol: ANG
  • Target Synonym: AI385586, ALS9, Ang1, Ang2, ANGIOGENIN, ANGIOGENIN 2, BRB, BRN, HEL168, MGC140149, MGC22466, MGC71966, RNASE4, RNASE5, Rnase5a
  • Entrez Gene Name: angiogenin, ribonuclease, RNase A family, 5
  • Hu Entrez ID: 283
  • Mu Entrez ID: 11727
  • Rat Entrez ID: 305843, 497229

    • 免疫原

    Human
    recombinant, human angiogenin

    应用

    ELISA (0.5-1 µg/ml)

    Immunoblotting (1-2 µg/ml)

    Neutralization Studies (EC₅₀= 100-150 µg/µg angiogenin, see comments)

    外形

    Lyophilized from 20 mM ammonium bicarbonate, PBS, 100 µg FAF-BSA.

    制备说明

    Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 1 mg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

    分析说明

    Positive Control
    HT-29 cells

    其他说明

    Shapiro, R. 1998. Biochemistry37, 6847.
    Soncin, F., et al. 1997. Biochem. Biophys. Res. Commun.236 604.
    Vallee, B.L. and Riordan, J.F. 1997. Cell Mol. Life Sci.53 803.
    Moenner, M., et al. 1994. Eur. J. Biochem.226 483.
    Hu, G.F., et al. 1993. PNAS.90 1217.
    Moroianu, J., et al. 1993. PNAS.90 3815.
    Soncin, F. 1992. PNAS.89 2232.
    Lee, F.S. and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun.161 121.
    Kurachi, K., et al. 1985. Biochemistry24, 5494.
    This antibody has been selected for its ability to neutralize the biological activity of human recombinant angiogenin. The activity of human recombinant angiogenin was measured based upon its ribonucleolytic activity toward yeast tRNA. Using the assay conditions described, 1 µg human recombinant angiogenin produces an absorbance change at 260 nm of ~2.0-3.0. The suggested antibody neutralization concentration required to yield 50% of the activity due to 1 µg of human recombinant angiogenin is approximately 100-150 µg of the antibody. The detection limit for human recombinant angiogenin by immunoblotting is ~5 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant angiogenin by ELISA is ~0.3 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    免责声明

    Toxicity: Standard Handling (A)

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    Alexandra Skorupa et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(15), 5024-5038 (2012-04-13)
    Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disorder affecting motoneurons. Mutations in angiogenin, encoding a member of the pancreatic RNase A superfamily, segregate with ALS. We previously demonstrated that angiogenin administration shows promise as a neuroprotective therapeutic in studies
    Marion C Hogg et al.
    Brain communications, 2(2), fcaa138-fcaa138 (2021-02-06)
    Loss-of-function mutations in the ribonuclease angiogenin are associated with amyotrophic lateral sclerosis. Angiogenin has been shown to cleave transfer RNAs during stress to produce 'transfer-derived stress-induced RNAs'. Stress-induced tRNA cleavage is preserved from single-celled organisms to humans indicating it represents
    Florian Pichot et al.
    Computational and structural biotechnology journal, 21, 401-417 (2023-01-10)
    Modification of tRNA is an integral part of the epitranscriptome with a particularly pronounced potential to generate diversity in RNA expression. Eukaryotic tRNA contains modifications in up to 20% of their nucleotides, but not all sites are always fully modified.
    Nikoletta A Gkatza et al.
    PLoS biology, 17(6), e3000297-e3000297 (2019-06-15)
    Posttranscriptional modifications in transfer RNA (tRNA) are often critical for normal development because they adapt protein synthesis rates to a dynamically changing microenvironment. However, the precise cellular mechanisms linking the extrinsic stimulus to the intrinsic RNA modification pathways remain largely

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