Anti-Orexin A (14-33) (Ab-1) Rabbit pAb

Purified rabbit polyclonal antibody. Recognizes orexin A. Supplied with a control peptide.

Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.

The hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.

This Anti-Orexin A (14-33) (Ab-1) Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunofluorescence for the detection of Orexin A (14-33) (Ab-1).

Human

a synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A

ELISA (see comments)
Frozen Sections (1-3 g/ml)
Immunofluorescence (1-4 g/ml)

Please refer to vial label for lot-specific concentration.

100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide.

Reconstitute peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Positive Control
Mouse or rat brain

De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell92, 437.
Sakurai, T., et al. 1998. Cell92, 573.
Schwartz, M.W. 1998. Nat. Med.4, 385.

This amino acid sequence is identical to orexin A from mouse, rat, and bovine. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with peptide at 10^-6 M. Unconjugated control peptide has been included for use as an internal control for antibody specificity. This antibody has been reported to work for ELISA. This antibody is also available in serum form, Anti-Orexin A (Ab-2) Rabbit pAb (Cat.No. PC362). Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)