Anti-P2X4 Receptor (373-388) Rabbit pAb

Purified rabbit polyclonal antibody. Recognizes the ~65 kDa P2X4 receptor protein.

Recognizes the ~65 kDa P2X4 receptor protein in mouse brain.

Rat

a synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH

Frozen Sections (1-2.5 g/ml)
Immunoblotting (5 g/ml)

Please refer to vial label for lot-specific concentration.

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Positive Control
Mouse brain, rat brain, or SK-N-SH cells

A protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10^-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.

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Toxicity: Standard Handling (A)