生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
同位素/亚型
IgG
运输
wet ice
储存温度
2-8°C
一般描述
Purified rabbit polyclonal antibody. Recognizes the ~65 kDa P2X4 receptor protein.
Recognizes the ~65 kDa P2X4 receptor protein in mouse brain.
免疫原
Rat
a synthetic peptide (KYVEDYEQGLSGEMNQ) corresponding to amino acids 373-388 of rat P2X4 receptor, conjugated to KLH
应用
Frozen Sections (1-2.5 g/ml)
Immunoblotting (5 g/ml)
包装
Please refer to vial label for lot-specific concentration.
外形
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
分析说明
Positive Control
Mouse brain, rat brain, or SK-N-SH cells
Mouse brain, rat brain, or SK-N-SH cells
其他说明
A protein database search confirms that this peptide sequence is unique to the P2X4 receptor protein. The signal was completely abolished in immunoblotting by preincubating the purified antibody with blocking peptide at 25 µg/ml. Immunohistochemistry was performed on floating mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M (approximately 2 µg/ml; data not shown). Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
Le, K.-T., et al. 1998. Neuroscience83, 177.
Nicke, A., et al. 1998. EMBO J.17, 3016.
Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev.50, 413.
North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol.7, 346.
Radford, K.M., et al. 1997. J. Neurosci.17, 6529.
Buell, G., et al. 1996. EMBO J.15, 55.
Collo, G., et al. 1996. J. Neurosci.16, 2495.
Seguela, P., et al. 1996. J. Neurosci.16, 448.
Bo, X., et al. 1995. FEBS Lett.375, 129.
Lewis, C., et al. 1995. Nature377, 432.
Nicke, A., et al. 1998. EMBO J.17, 3016.
Ralevic, V. and Burnstock, G. 1998. Pharmcolol. Rev.50, 413.
North, R.A. and Barnard, E.A. 1997. Curr. Opin. Neurobiol.7, 346.
Radford, K.M., et al. 1997. J. Neurosci.17, 6529.
Buell, G., et al. 1996. EMBO J.15, 55.
Collo, G., et al. 1996. J. Neurosci.16, 2495.
Seguela, P., et al. 1996. J. Neurosci.16, 448.
Bo, X., et al. 1995. FEBS Lett.375, 129.
Lewis, C., et al. 1995. Nature377, 432.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
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Hongxia Chen et al.
Molecular medicine reports, 13(1), 697-704 (2015-12-10)
P2X4 receptor (P2X4R) is the most widely expressed subtype of the P2XRs in the purinergic receptor family. Adenosine triphosphate (ATP), a ligand for this receptor, has been implicated in the pathogenesis of asthma. ATP‑P2X4R signaling is involved in pulmonary vascular
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