PC711
Anti-CHIP Rabbit pAb
liquid, Calbiochem®
别名:
Anti-Carboxyl Terminus of Hcs70-Interacting Protein
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
Drosophila, hamster, rat, human, monkey, mouse
Application:
—
Citations:
16
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% Sodium azide as preservative
species reactivity
Drosophila, hamster, rat, human, monkey, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.
Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.
Immunogen
a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence
Application
Immunoblotting (1:200-1:1000)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
Immunofluorescence (1:250)
Immunoprecipitation (1:20)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
COS-7 cells
COS-7 cells
Other Notes
May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable