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PSHT010R

Millicell® 24孔板

pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible, pack of 1

别名:

细胞培养板

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UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006
Material:
polycarbonate membrane
polystyrene plate
Pore size:
0.4 μm
Sterility:
sterile
γ-irradiated
Binding type:
Tissue Culture (TC)-treated surface

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产品名称

Millicell® 24孔板, pore size 0.4 μm, polycarbonate membrane, sterile, Tissue Culture (TC)-treated surface, automation compatible, pack of 1

material

polycarbonate membrane
polystyrene plate

description

components: 24-well cell culture plate (1), single well feeder tray (1), 24-well receiver plate (1), lid (2)

sterility

sterile
γ-irradiated

feature

lid
 100x106 pores/cm2 (pore density)
 automation compatible

packaging

pack of 1

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

L × W × H

127.76 mm × 85.47 mm × 20.32 mm, with lid

filter diam.

9.5 mm

filtration area

0.7 cm2

plate size

24 wells

working volume

0.4 mL

matrix

Isopore™

Quality Level

400

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

相关类别

General description

Millicell细胞培养嵌套板是一种专利装置，用于支持内皮和上皮细胞系（包括CaCo-2）的细胞生长和分化。

Millicell装置有0.4 µm PCF和多种PET薄膜孔径。培养板给用户提供了最大方便性，有许多特点，例如更加方便移液的上侧辅助孔以及下侧接触孔。为了降低污染细胞单层的风险，薄膜板有支脚，使板子在从饲养托盘拆下时高于工作表面。泪滴形状的容纳孔消除了在组装板子时产生的气泡。

Millicell培养板经优化用于生长细胞和维持细胞单层的高度完整性。Millicell培养板薄膜上生长的细胞的生长情况比在塑料上好，因为细胞从上侧和下侧均获得营养。更加密切地模拟了体内细胞生长和功能。Millicell培养板设计用于分析及细胞生长，亦可用作手动或自动细胞接种、饲养和清洗系统。

使用这一完善的系统，您可以在一个以薄膜做底的培养板上生长、饲养和分析细胞。培养板可以与单槽或多孔饲养托盘配用。在进行转运分析时，只需将培养板转换成分析用的多孔转运托盘。

这种流线型设计增强了下列功能：

-接种和饲养系统

-自动液体处理系统和跨距移液

-下侧和上侧均可接触到细胞

-经上皮电阻（TEER）测量系统

Application

Millicell®-24 Cell Culture Insert Plate, polycarbonate, 0.4 μm has been used for seeding human leukemia monocytic cells and human colorectal adenocarcinoma cells for differentiation. It has also been used to study drug transport across Caco-2 monolayers.

Features and Benefits

  • Enhanced Cell Growth: Cells cultured on Millicell plates exhibit better growth compared to traditional plastic, as they receive nutrients from both the apical and basolateral sides, mimicking in vivo conditions.
  • User-Friendly Design: Incorporates apical assist for easy pipetting and basolateral access. Tear drop-shaped receiver wells prevent air bubbles during assembly.
  • Streamlined Workflow: The complete system allows for simultaneous growth, feeding, and analysis within one membrane-bottom plate, simplifying workflows.
  • Automation Compatible: Designed to work seamlessly with seed-and-feed systems, automated liquid handling, and TEER measurement systems, enhancing laboratory efficiency.

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Zsolt Sáfár et al.
Journal of pharmaceutical sciences, 107(11), 2742-2747 (2018-07-29)
Breast cancer resistance protein (BCRP) is a point of interest in drug-drug interaction safety testing. Therefore, a consensus probe that can be applied as victim in multiple experimental settings is of great benefit. Identification of candidates has been driven by
Daniel Wai-Hung Ho et al.
Nature communications, 12(1), 3684-3684 (2021-06-19)
Interaction between tumor cells and immune cells in the tumor microenvironment is important in cancer development. Immune cells interact with the tumor cells to shape this process. Here, we use single-cell RNA sequencing analysis to delineate the immune landscape and
Ivan Luzardo-Ocampo et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 146, 111835-111835 (2020-11-02)
Bioactive compounds from food products made from natural ingredients such as corn and common bean could target the NLRP3 inflammasome, protein scaffolds with a key role in the moderation of intestinal inflammation. This research aimed to evaluate the anti-inflammatory effect

实验方案

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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