GLUTag Mouse Colonic Endocrine Cell Line

Glucagon-like peptide-1 (GLP-1), an important regulator of glucose dependent insulin secretion, is a peptide hormone produced by enteroendocrine cells.² It also plays a role in food intake and gut motility. GLP-1 activity is preserved in individuals with type 2 diabetes, allowing it to be an effective target for drugs to treat diabetes and obesity.

The GLUTag cell line is an effective intestinal endocrine L-cell model derived from colonic tumors of transgenic mice. This cell line secretes a variety of pro-glucagon derived peptides (PGDPs) such as GLI, IRG, and GLP-1.¹ GLUTag cells are among one of the most widely used cell models to understand GLP-1 secretion. GLUTag cells are capable of activating GLP-1 secretion in vivo and have a phenotype that closely mirrors non-immortalized intestinal L-cells with some slight genetic differences. The immunoreactive profile of GLUTag cells also suggests L-cell lineage by being positively immunoreactive to GLP-1 and cholecystokinin, but negative for a large variety of other hormones such as calcitonin, insulin, and gastrin.

Although the GLUTag cell line has murine origins, they have been shown to have a relevant response mechanism that essentially mirrors the GLP-1 secretion response in humans. This means that testing potential therapeutic agents using GLUTag cells has been shown to still have a strong relevance to a human model. The ease-of-use of GLUTag cells becomes quite invaluable when compared to the difficulties of using primary human L-cells.

Source
Derived from an endocrine carcinoma in a mouse expressing SV40 Large T antigen. Fragments of the tumors were subcutaneously propagated in nude mice and individual cells from one of the tumors was isolated and single-cell cloned.

References
1. Drucker DJ, Jin T, Sylvia L, Young TA, Brubaker PL. 1994. Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line. Molecular Endocrinology. 8(12):1646–1655.
2. Kuhre RE, Wewer Albrechtsen NJ, Deacon CF, Balk-Møller E, Rehfeld JF, Reimann F, Gribble FM, Holst JJ. 2016. Peptide production and secretion in GLUTag, NCI-H716, and STC-1 cells: a comparison to native L-cells. Journal of Molecular Endocrinology. 56(3):201–211.

GLUTag cells are murine intestinal endocrine L-cells derived from colonic tumors. They secrete a variety of pro-glucagon derived peptides (PGDPs) such as GLI, IRG, and GLP-1. GLUTag cells are among one of the most widely used cell models to understand GLP-1 secretion.

GLUTag cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

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