Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.

Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.

This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.

Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.

Human

a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1

Immunoblotting (1:1000)

Please refer to vial label for lot-specific concentration.

Toxicity: Highly Toxic (H)

In Tris-Citrate/phosphate, pH 7.0-8.0.

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Positive Control
Jurkat or NIH3T3 cells

Antibody should be titrated for optimal results in individual systems.

Mertens, A.E., et al. 2003. FEBS Lett.546, 11.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.

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