产品名称
神经氨酸酶（唾液酸酶）, from Arthrobacter ureafaciens
biological source
bacterial (Arthrobacter ureafaciens)
form
solution
specific activity
~25 units/mg protein
packaging
pkg of 1 U (100 μl)
manufacturer/tradename
Roche
optimum pH
5.0-5.5
storage temp.
2-8°C
Quality Level
Application
- 细胞表面凝集素阵列分析。
- 血细胞凝集试验。
- 细胞粘附试验。
对于糖脂的水解，需要表面活性剂的存在。由于具有广泛的底物特异性，该酶非常适合从各种生物材料的糖缀合物中完全去除唾液酸。
神经氨酸酶已用于：
- 检测人间变性大细胞淋巴瘤细胞中的细胞表面糖基化
- 从细胞中释放的唾液酸
- 抗体覆盖凝集素微阵列
Biochem/physiol Actions
可切割与Gal、GlcNAc、GalNAc、AcNeu、GlcNeu、寡糖、糖脂或糖蛋白连接的α2,3-、α2,6-或α2,8-末端唾液酸残基。其切割的相对速率为α2,6 >α2,3 >α2,8，该结果在三糖和四糖中的化学键上进行测定。
General description
神经氨酸酶可水解通过α2,3-、α2,6-或α2,8-连接至寡糖、多糖、粘多糖、糖蛋白和糖脂的N-或O-酰基神经氨酸末端（水解率：α2,6 > α2,3 > α2,8）。需要注意的是，水解糖脂需要存在表面活性剂。由于具有广泛的底物特异性，该酶非常适合从各种生物材料的糖缀合物中完全去除唾液酸。
该产品是具有以下分子量的同工酶（L，M1，M2和S）的混合物： ~52 kDa、66 kDa和88 kDa。
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
溶于10 mM磷酸钠、0.1％Micr-O-Protect（w/v）、0.25 mg/ml牛血清白蛋白(pH 7)溶液中
Preparation Note
工作浓度：酶/底物比率应在0.04U/25-80 μg范围内。
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
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实验方案
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
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