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生物来源
chicken egg white
质量水平
方案
≥95%
表单
solid
比活
>23000 U/mg (Shugar units)
分子量
14.4 kDa
包装
pkg of 10 g
制造商/商品名称
Roche
最佳pH
6.0-7.0
吸附
3.9 at 280 nm (10 mg enzyme/ml)
一般描述
溶菌酶,粘肽乙酰胞壁质聚糖水解酶
应用
溶菌酶已用于监测实时PCR的转录过程和样品制备。
溶菌酶已用于:
- 细胞壁降解
- 原生质体的制备
- 溶菌
- 药理学(对粘膜的抗炎作用;组织愈合;防止出血;粘液分泌正常化;热原溶解;增强抗生素作用)
- 食品和饮料(增味剂)
- 分离核酸前的样品制备
生化/生理作用
溶菌酶水解切割粘多糖和肽聚糖相应聚糖骨架(后者是与肽链连接或交联的糖胺聚糖)中N-乙酰-D-葡糖胺与N-乙酰胞壁酸残基(GlcNAc β1-4Mur)之间的键。溶菌酶能有效破坏细菌的细胞壁。
单位定义
一个Shugar单位是指:在+25°C下,在1 ml pH7.0的溶壁微球菌( Micrococcus luteus )悬浮液中,在450nm波长处的初始吸光度为0.750,路径长度为10mm,引起吸光度以每分钟0.001的速率降低的酶量。
制备说明
工作溶液:盐酸溶菌酶易溶于水和缓冲溶液,但不溶于有机溶剂。
储存条件(工作溶液):-15至-25°C
盐酸溶菌酶水溶液(2mg/ml,溶剂为蒸馏水)可在2至8℃下储存数天,或在-15至-25℃下储存数周。
储存条件(工作溶液):-15至-25°C
盐酸溶菌酶水溶液(2mg/ml,溶剂为蒸馏水)可在2至8℃下储存数天,或在-15至-25℃下储存数周。
重悬
使用双蒸水制备浓度为50 mg/ml的储备溶液。将储备溶液分装并保存在-15至-25°C。分装品用后丢弃。
储存及稳定性
2至8 °C储存。(干燥储存!)
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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