biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12CA5, monoclonal
assay
≥95% (HPLC and SDS-PAGE)
form
solution
packaging
pkg of 50 μg (500 μl)
manufacturer/tradename
Roche
concentration
0.1 mg/mL
isotype
IgG2bκ
epitope sequence
YPYDVPDYA
storage temp.
−20°C
General description
抗HA-过氧化物酶是与过氧化物酶偶联的HA肽的单克隆抗体。抗HA-过氧化物酶可识别源自人流感病毒血凝素蛋白（氨基酸98-106）的HA九肽序列（YPYDVPDYA）。即使通过称为“表位标记”的技术将HA肽表位引入不相关的重组蛋白中，抗体也可识别其抗原决定簇。
Immunogen
来自人流感病毒血凝素蛋白的氨基酸98-106
Application
- 通过以下方法，使用抗HA过氧化酶对天然流感血凝素蛋白和重组HA标签蛋白进行免疫化学检测：
- 斑点杂交法
- 蛋白质印迹法
注释：对于灵敏度不重要的实验，请使用抗HA过氧化酶（12CA5）。在低10倍浓度的蛋白质印迹中，为获得更高灵敏度的检测，请使用抗-HA高亲和力。
Biochem/physiol Actions
抗HA-过氧化物酶可识别来自人流感血凝素（HA）蛋白的9-氨基酸序列YPYDVPDYA。无论该表位位置如何（N末端、C末端或内部），其也可在融合蛋白中被识别。
该抗体与HA表位反应，HA表位是衍生自流感血凝素蛋白的九肽序列（YPYDVPDYA）。
Preparation Note
稳定剂：BSA，蔗糖，0.02% 百里酚
工作浓度：偶联物的工作浓度取决于应用和底物。
以下浓度应作为使用指南：
工作溶液：Western封闭试剂在1x PBS中的20倍稀释液。
工作浓度：偶联物的工作浓度取决于应用和底物。
以下浓度应作为使用指南：
- 蛋白质印迹法：0.1 μg/ml
工作溶液：Western封闭试剂在1x PBS中的20倍稀释液。
Analysis Note
通过测试每批抗HA抗体相对于标准品的功能性和纯度，以确认每个新试剂制剂的质量。
Other Notes
仅用于生命科学研究。不可用于诊断。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
低风险生物材料
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