产品名称
甲酰胺, =99%, liquid, pH 4-10 (20 °C, 200 g/L), suitable for hybridization
SMILES string
NC=O
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
assay
99%
form
liquid
autoignition temp.
932 °F
mol wt
45.04
packaging
pkg of 500 mL
manufacturer/tradename
Roche
technique(s)
hybridization: suitable
color
colorless
refractive index
n20/D 1.447 (lit.)
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
density
1.134 g/mL at 25 °C (lit.)
absorption
≤0.08 at 290 nm
shipped in
wet ice
storage temp.
2-8°C
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Analysis Note
污染物：≤5ppm重金属(比如Pb），≤5ppm Fe
Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。
甲酰胺已用于：
原位杂交
原位杂交
- 用作RNA聚丙烯酰胺凝胶电泳的2X变性上样染料组分
- 在血管渗漏试验中，提取小鼠后肢的伊文思蓝染料
- 用作裂解和聚腺苷酸化活性试验所用的终止缓冲液组分
General description
甲酰胺是一种有机溶剂，能使核酸在室温下变性和复性。它尤其适合用在反应时间长以及高温通过断链和脱嘌呤作用等损害生物活性的实验中。甲酰胺降低了双链核酸的热稳定性，通常用于DNA复性或DNA-RNA杂交。甲酰胺浓度和反应温度决定了反应的特异性和速率。
Other Notes
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
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