产品名称
抗 HA-过氧化物酶，高亲和力, from rat IgG1
biological source
rat
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 3F10, monoclonal
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 25 U (25 μg)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Quality Level
Application
- 使用抗 HA-过氧化物酶，高亲和力检测 HA 标记的重组蛋白：ELISA
- 蛋白质印迹
Biochem/physiol Actions
抗 HA-过氧化物酶，高亲和力（3F10）识别来自人流感血凝素（HA）蛋白的 9-氨基酸序列YPYDVPDYA。
无论其位置如何（N 末端，C 末端或内部），该表位也在融合蛋白中被识别。
无论其位置如何（N 末端，C 末端或内部），该表位也在融合蛋白中被识别。
General description
抗 HA-过氧化物酶，高亲和力是 HA-肽（克隆 3F10）的单克隆抗体，与过氧化物酶缀合。
The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:
- The size, cellular localization, and abundance of proteins produced by newly discovered genes.
- Post-translational modifications of proteins.
- The movement of proteins within cell membranes.
- The identity of proteins within functional protein complexes.
- The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.
Immunogen
该表位由来自人流感病毒血凝素蛋白的氨基酸 98-106 组成。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
稳定剂：蛋白质稳定剂
工作浓度：缀合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
工作浓度：缀合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
- 斑点印迹：50 mU/ml
- ELISA：25 mU/ml
- 蛋白质印迹：50 mU/ml
加入 1.0 ml 双蒸水，最终浓度为 25 U/mL。
使用前再水化 10 分钟。
使用前再水化 10 分钟。
未找到合适的产品？
试试我们的产品选型工具.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Ranran Wang et al.
Cancers, 12(6) (2020-06-25)
The epigenetic reader BRD4 binds acetylated histones and plays a central role in controlling cellular gene transcription and proliferation. Dysregulation of BRD4's activity has been implicated in the pathogenesis of a wide variety of cancers. While blocking BRD4 interaction with
Julia K Günther et al.
Biology, 9(5) (2020-05-21)
Rigosertib, via reactive oxygen species (ROS), stimulates cJun N-terminal kinases 1/2 (JNK1/2), which inactivate RAS/RAF signaling and thereby inhibit growth and survival of tumor cells. JNK1/2 are not only regulated by ROS-they in turn can also control ROS production. The
Regina Gratz et al.
The New phytologist, 225(1), 250-267 (2019-09-06)
The key basic helix-loop-helix (bHLH) transcription factor in iron (Fe) uptake, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT), is controlled by multiple signaling pathways, important to adjust Fe acquisition to growth and environmental constraints. FIT protein exists in active and inactive
Shiori Akabane et al.
The Journal of biological chemistry, 291(31), 16162-16174 (2016-06-16)
Phosphatase and tensin homolog-induced putative kinase 1 (PINK1), a Ser/Thr kinase, and PARKIN, a ubiquitin ligase, are causal genes for autosomal recessive early-onset parkinsonism. Multiple lines of evidence indicate that PINK1 and PARKIN cooperatively control the quality of the mitochondrial
DongHyuk Lee et al.
Nature communications, 11(1), 1838-1838 (2020-04-17)
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持