生物来源
rat
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
clone 3F10, monoclonal
表单
lyophilized (clear, colorless solution after reconstitution)
包装
pkg of 25 U (25 μg)
制造商/商品名称
Roche
同位素/亚型
IgG1
表位序列
YPYDVPDYA
储存温度
2-8°C
一般描述
抗 HA-过氧化物酶，高亲和力是 HA-肽（克隆 3F10）的单克隆抗体，与过氧化物酶缀合。
The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:
- The size, cellular localization, and abundance of proteins produced by newly discovered genes.
- Post-translational modifications of proteins.
- The movement of proteins within cell membranes.
- The identity of proteins within functional protein complexes.
- The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.
免疫原
该表位由来自人流感病毒血凝素蛋白的氨基酸 98-106 组成。
应用
- 使用抗 HA-过氧化物酶，高亲和力检测 HA 标记的重组蛋白：ELISA
- 蛋白质印迹
生化/生理作用
抗 HA-过氧化物酶，高亲和力（3F10）识别来自人流感血凝素（HA）蛋白的 9-氨基酸序列YPYDVPDYA。
无论其位置如何（N 末端，C 末端或内部），该表位也在融合蛋白中被识别。
无论其位置如何（N 末端，C 末端或内部），该表位也在融合蛋白中被识别。
制备说明
稳定剂：蛋白质稳定剂
工作浓度：缀合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
工作浓度：缀合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
- 斑点印迹：50 mU/ml
- ELISA：25 mU/ml
- 蛋白质印迹：50 mU/ml
加入 1.0 ml 双蒸水，最终浓度为 25 U/mL。
使用前再水化 10 分钟。
使用前再水化 10 分钟。
其他说明
仅用于生命科学研究。不可用于诊断。
未找到合适的产品？
试试我们的产品选型工具.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
此项目有
Regina Gratz et al.
The New phytologist, 225(1), 250-267 (2019-09-06)
The key basic helix-loop-helix (bHLH) transcription factor in iron (Fe) uptake, FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT), is controlled by multiple signaling pathways, important to adjust Fe acquisition to growth and environmental constraints. FIT protein exists in active and inactive
Julia K Günther et al.
Biology, 9(5) (2020-05-21)
Rigosertib, via reactive oxygen species (ROS), stimulates cJun N-terminal kinases 1/2 (JNK1/2), which inactivate RAS/RAF signaling and thereby inhibit growth and survival of tumor cells. JNK1/2 are not only regulated by ROS-they in turn can also control ROS production. The
Ranran Wang et al.
Cancers, 12(6) (2020-06-25)
The epigenetic reader BRD4 binds acetylated histones and plays a central role in controlling cellular gene transcription and proliferation. Dysregulation of BRD4's activity has been implicated in the pathogenesis of a wide variety of cancers. While blocking BRD4 interaction with
Satoshi Hashimoto et al.
Scientific reports, 10(1), 3422-3422 (2020-02-27)
Ribosome stalling triggers the ribosome-associated quality control (RQC) pathway, which targets collided ribosomes and leads to subunit dissociation, followed by proteasomal degradation of the nascent peptide. In yeast, RQC is triggered by Hel2-dependent ubiquitination of uS10, followed by subunit dissociation
Jihoon Ha et al.
International journal of molecular sciences, 21(9) (2020-05-02)
p62/sequestosome-1 is a scaffolding protein involved in diverse cellular processes such as autophagy, oxidative stress, cell survival and death. It has been identified to interact with atypical protein kinase Cs (aPKCs), linking these kinases to NF-κB activation by tumor necrosis
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持