biological source
Aspergillus niger
form
lyophilized
specific activity
6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
mol wt
97 kDa
packaging
pkg of 3,500 U (11202367001), pkg of 500 U (11202332001)
manufacturer/tradename
Roche
optimum pH
4.6-4.8
storage temp.
2-8°C
Quality Level
General description
淀粉葡萄糖苷酶是一种 a 1,4-α-葡萄糖水解酶,同时也是一种双糖型α葡萄糖苷酶。它是由多种曲霉属微生物合成的。这种胞外酶是主要的工业酶之一。通过固定化可以增强淀粉葡萄糖苷酶的稳定性。
Application
来自黑曲霉的淀粉葡萄糖苷酶可用于测定淀粉。
Biochem/physiol Actions
可水解与多个葡萄糖单元的寡糖或多糖 α1,4-或 α1,6-连接的末端葡萄糖。本产品是 D-葡萄糖。
来自黑曲霉(Aspergillus niger)的淀粉葡萄糖苷酶能够水解淀粉和麦芽寡糖的 α-D-(1-4) 和 α-(1,6)-糖苷键。淀粉葡萄糖苷酶是一种胞外酶,可以将淀粉转化成糊精和葡萄糖。在淀粉加工工业中该酶用于由玉米糖浆商业生产D-葡萄糖。
Preparation Note
工作浓度:缀合物的工作浓度取决于应用和底物。
以下浓度可作为指导:
工作溶液:含有 0.1% Tween 20 的 Tris 缓冲盐水。
储存条件(工作溶液):2 至 8°C
以下浓度可作为指导:
- 蛋白质印迹:1 至 10 μg/ml
工作溶液:含有 0.1% Tween 20 的 Tris 缓冲盐水。
储存条件(工作溶液):2 至 8°C
Other Notes
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
Boaz Hilman et al.
Plant, cell & environment, 44(8), 2522-2535 (2021-06-08)
Little is known about the sources and age of C respired by tree roots. Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool
The action of an amyloglucosidase of Aspergillus niger on starch and malto-oligosaccharides.
J H PAZUR et al.
The Journal of biological chemistry, 234(8), 1966-1970 (1959-08-01)
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