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Merck
CN

16669

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

别名:

5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal

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About This Item

经验公式(希尔记法):
C14H15BrClNO6
CAS Number:
分子量:
408.63
Beilstein:
1552602
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:

等级

Molecular Biology

质量水平

无菌性

non-sterile

方案

≥98.0% (HPLC)

表单

powder

溶解性

DMF: soluble
DMSO: soluble

适用性

suitable for β-galactosidase test

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1

InChI key

CHRVKCMQIZYLNM-RKQHYHRCSA-N

一般描述

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

应用

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

原理

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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