16669
Magenta-Gal
reagent for selection of recombinant bacterial clones
别名:
5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
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关于此项目
经验公式（希尔记法）:
C14H15BrClNO6
化学文摘社编号:
分子量:
408.63
Beilstein:
1552602
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
等级
Molecular Biology
质量水平
无菌性
non-sterile
方案
≥98.0% (HPLC)
表单
powder
溶解性
DMF: soluble
DMSO: soluble
适用性
suitable for β-galactosidase test
储存温度
−20°C
SMILES字符串
OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
InChI key
CHRVKCMQIZYLNM-RKQHYHRCSA-N
一般描述
Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
应用
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.
生化/生理作用
When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and
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