G6639
异硫氰酸胍
≥97% (titration)
别名:
硫氰酸亚脲, 硫氰酸胍
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关于此项目
线性分子式:
NH2C(=NH)NH2 · HSCN
化学文摘社编号:
分子量:
118.16
UNSPSC Code:
12352209
NACRES:
NA.55
PubChem Substance ID:
EC Number:
209-812-1
Beilstein/REAXYS Number:
3563461
MDL number:
biological source
synthetic
assay
≥97% (titration)
form
powder or crystals
technique(s)
RNA extraction: suitable
color
white to off-white
mp
120-122 °C (lit.)
solubility
water: 4 M, clear, colorless
anion traces
chloride (Cl-): ≤2.5%
Quality Level
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Application
硫氰酸胍可用于：
- 用作变性剂研究其对球状蛋白表面活性的影响
- 从原代肝细胞提取总核糖核酸(RNA)
- 用作从人骨中提取DNA的培养缓冲液组分
- 用作提取象鼻鲨的DNA的硫氰酸胍(GITC)裂解缓冲液组分
- 用作从大鼠胰腺组织提取RNA的裂解液组分
离液剂和强效变性剂;溶解细胞。
Biochem/physiol Actions
硫氰酸胍(GTC)是一种强效蛋白质变性剂，能够变性内源性核糖核酸酶。它在从核糖体中分离核蛋白体核糖核酸(rRNA)方面起着重要作用。GTC可与苯酚、氯仿联用，从细胞和组织中分离总核糖核酸。
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
DNA fromMycobacterium tuberculosisIdentified in Mediaeval Human Skeletal Remains Using Polymerase Chain Reaction
Taylor M G, et al.
Journal of Archaeological Science, 23, 789-798 (1996)
Emilia Bramanti et al.
Analytical biochemistry, 351(1), 100-113 (2006-01-28)
In this article, a multidimensional dynamic surface tension detector (DSTD), in a parallel configuration with a UV-visible diode array absorbance detector, is presented in a novel flow injection analysis (FIA) application to study the effects of chemical denaturants urea, guanidinium
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates.
Julian R Peat et al.
F1000Research, 6, 526-526 (2017-06-06)
Methylation of CG dinucleotides constitutes a critical system of epigenetic memory in bony vertebrates, where it modulates gene expression and suppresses transposon activity. The genomes of studied vertebrates are pervasively hypermethylated, with the exception of regulatory elements such as transcription
Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique
Soni N
PharmaTutor, 4, 20-22 (2016)
Angela Molinaro et al.
Cell metabolism, 29(6), 1400-1409 (2019-04-16)
Phosphatidylinositol-3-kinase (PI3K) activity is aberrant in tumors, and PI3K inhibitors are investigated as cancer therapeutics. PI3K signaling mediates insulin action in metabolism, but the role of PI3K isoforms in insulin signaling remains unresolved. Defining the role of PI3K isoforms in
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