U1250
尿素
≥99.5%, pellets, ReagentPlus®
别名:
碳酰二胺, 碳酰胺
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关于此项目
线性分子式:
NH2CONH2
化学文摘社编号:
分子量:
60.06
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
200-315-5
MDL number:
Beilstein/REAXYS Number:
635724
Assay:
≥99.5%
Form:
pellets
产品名称
尿素, ReagentPlus®, ≥99.5%, pellets
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
SMILES string
NC(N)=O
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
product line
ReagentPlus®
assay
≥99.5%
form
pellets
Quality Level
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
solubility
H2O: 8 M
density
1.335 g/mL at 25 °C (lit.)
functional group
amine
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General description
尿素是离液剂，用于蛋白质变性。它扰乱了蛋白质的二级、三级和四级结构中的氢键。尿素还可以干扰 DNA 二级结构中存在的氢键。
Application
尿素已用作蛋白质变性剂。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Christos D Georgiou et al.
Redox biology, 17, 128-142 (2018-04-24)
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
Sreenivasa C Ramaiahgari et al.
Archives of toxicology, 88(5), 1083-1095 (2014-03-07)
Immortalized hepatocyte cell lines show only a weak resemblance to primary hepatocytes in terms of gene expression and function, limiting their value in predicting drug-induced liver injury (DILI). Furthermore, primary hepatocytes cultured on two-dimensional tissue culture plastic surfaces rapidly dedifferentiate
实验方案
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
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