RDD035
HEPES钠盐 钠盐
≥99.5% (titration), free-flowing, Redi-Dri™
别名:
4-(2-羟乙基)哌嗪-1-乙磺酸 钠盐, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸) 钠盐
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关于此项目
经验公式（希尔记法）:
C8H17N2NaO4S
化学文摘社编号:
分子量:
260.29
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
产品名称
HEPES钠盐 钠盐, ≥99.5% (titration), free-flowing, Redi-Dri™
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
product line
Redi-Dri™
assay
≥99.5% (titration)
quality
free-flowing
useful pH range
6.8-8.2
pKa (25 °C)
7.5
application(s)
diagnostic assay manufacturing
Quality Level
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Physical form
冻干、喷雾干燥或结晶的材料。
Application
HEPES sodium salt can be used:
- as a protein precipitation buffer
- in cell culture media
- as a binding buffer
Features and Benefits
- Redi-Dri™ eliminates clumping for hygroscopic chemicals
- powder remains free-flowing and easy to use
- Safer to handle and weigh out for standard use in lab
- Eliminates clumps, maintains product moisture, quality, and characteristics
General description
HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
J R Eidet et al.
Experimental eye research, 94(1), 109-116 (2011-12-14)
The use of amniotic membrane (AM) represents one of the major developments in ocular surface reconstruction. However, in a study on patients with primary pterygium, transplantation of AM with ex vivo expanded human conjunctival epithelial cells (HCjE) promoted earlier epithelialization
商品
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
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