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04529

腈水解酶

Name: 腈水解酶
Brand: SIGMA

recombinant, expressed in E. coli, ≥2.0 U/mg

别名:

Nitrile aminohydrolase

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关于此项目

化学文摘社编号:

9024-90-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC 号:

3.5.5.1 (BRENDA, IUBMB)

MDL number:

MFCD04040969

Specific activity:

≥2.0 U/mg

Recombinant:

expressed in E. coli

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recombinant

expressed in E. coli

form

crystals, powder

specific activity

≥2.0 U/mg

mol wt

41 kDa

storage temp.

2-8°C

General description

腈水解酶属于C-N水解酶超家族。它具有催化三联体基序：谷氨酸，赖氨酸和半胱氨酸，分布在真菌，植物和动物中。该蛋白的等电点（pI）为8.1。天然聚丙烯酰胺凝胶电泳（PAGE）。

Application

腈水解酶可用于丙烯酰基交联纤维素二醛（ACCD）的固定化，用于催化活性研究。

Biochem/physiol Actions

腈水解酶催化氨的释放和腈形成羧酸。腈水解酶的寡聚结构组织对其功能至关重要。它显示出广泛的底物特异性。

Packaging

无底玻璃瓶。内含物装在插入的融合锥内。

Other Notes

1U对应于在pH 7.2和25℃下每分钟释放1 μmol氨且丙烯腈转化为丙烯酸的酶的量

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

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Bioconversion of acrylonitrile to acrylic acid by rhodococcus ruber strain AKSH-84.

Ahmed Kamal et al.

Journal of microbiology and biotechnology, 21(1), 37-42 (2011-02-09)

A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was

Enhanced catalytic activity of new acryloyl crosslinked cellulose dialdehyde-nitrilase Schiff base and its reduced form for nitrile hydrolysis.

Shivani Jamwal et al.

International journal of biological macromolecules, 131, 117-126 (2019-03-08)

Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized

Crystallographic analysis of a thermoactive nitrilase.

Joanna E Raczynska et al.

Journal of structural biology, 173(2), 294-302 (2010-11-26)

The nitrilase superfamily is a large and diverse superfamily of enzymes that catalyse the cleavage of various types of carbon-nitrogen bonds using a Cys-Glu-Lys catalytic triad. Thermoactive nitrilase from Pyrococcus abyssi (PaNit) hydrolyses small aliphatic nitriles like fumaro- and malononitryl.

Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid.

Zhi-Qiang Liu et al.

PloS one, 8(6), e67197-e67197 (2013-07-05)

In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase

Biochemical and mutational studies of the Bacillus cereus CECT 5050T formamidase support the existence of a C-E-E-K tetrad in several members of the nitrilase superfamily.

Pablo Soriano-Maldonado et al.

Applied and environmental microbiology, 77(16), 5761-5769 (2011-06-28)

Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate

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全球贸易项目编号

货号	GTIN
238430-10G	04061838347695
238430-50G	04061838786890
04529-50MG	04061833227930
04529-10MG	04061833227923

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