一般描述
腈水解酶属于C-N水解酶超家族。它具有催化三联体基序：谷氨酸，赖氨酸和半胱氨酸，分布在真菌，植物和动物中。该蛋白的等电点（pI）为8.1。天然聚丙烯酰胺凝胶电泳（PAGE）。
应用
腈水解酶可用于丙烯酰基交联纤维素二醛（ACCD）的固定化，用于催化活性研究。
生化/生理作用
腈水解酶催化氨的释放和腈形成羧酸。腈水解酶的寡聚结构组织对其功能至关重要。它显示出广泛的底物特异性。
包装
无底玻璃瓶。内含物装在插入的融合锥内。
其他说明
1U对应于在pH 7.2和25℃下每分钟释放1 μmol氨且丙烯腈转化为丙烯酸的酶的量
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Y V D Nageshwar et al.
Bioprocess and biosystems engineering, 34(5), 515-523 (2010-12-29)
Microbial nitrilases are biocatalysts of interest and the enzyme produced using various inducers exhibits altered substrate specificity, which is of great interest in bioprocess development. The aim of the present study is to investigate the nitrilase-producing Alcaligenes faecalis MTCC 10757
Zhi-Qiang Liu et al.
PloS one, 8(6), e67197-e67197 (2013-07-05)
In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase
Zhi-Jun Zhang et al.
Journal of biotechnology, 152(1-2), 24-29 (2011-01-26)
The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high concentrations (>300mM), which resulted in a low yield of the target product (R)-(-)-mandelic acid. To relieve
Pablo Soriano-Maldonado et al.
Applied and environmental microbiology, 77(16), 5761-5769 (2011-06-28)
Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate
Ahmed Kamal et al.
Journal of microbiology and biotechnology, 21(1), 37-42 (2011-02-09)
A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持