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Merck
CN

11557

2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸) 二铵盐

chromogenic, ≥98% (HPLC), powder or crystals

别名:

2,2′-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐, AzBTS-(NH4)2

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关于此项目

经验公式(希尔记法):
C18H24N6O6S4
化学文摘社编号:
分子量:
548.68
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
250-396-6
MDL number:
Beilstein/REAXYS Number:
7329461
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产品名称

2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸) 二铵盐, ≥98% (HPLC)

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key

OHDRQQURAXLVGJ-AXMZSLBLSA-N

SMILES string

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

assay

≥98% (HPLC)

form

powder or crystals

impurities

≤6.0% water

solubility

H2O: 10 mg/mL, slightly hazy to clear

storage temp.

2-8°C

Quality Level

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Application

2,2′-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)联胺盐可用于:
  • 通过自由基清除法测试赤松树皮(PDB)提取物的抗氧化特性
  • 测定芦荟样本的抗氧化性(AOC)
  • 在酶联免疫吸附测定(ELISA)中用作链霉亲和素-辣根过氧化物酶(HRPO)的底物

2,2′-联氮基-双3-乙基苯并噻唑啉-6-磺酸)是一种过氧化物酶底物,适用于ELISA程序。该底物产生一种可溶性最终产物,其颜色为绿色,可在405nm分光光度法下读取。用1%十二烷基硫酸钠(SDS)可使反应停止。 推荐用于ELISA (微孔)程序,不推荐用于膜应用。

Other Notes

过氧化物酶的显色底物。酶免疫分析(EIA)中的ABTS-H2O2-过氧化物酶系统

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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分析证书(COA)

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A Szutowicz et al.
Analytical biochemistry, 138(1), 86-94 (1984-04-01)
Monoamine oxidase is assayed in tissue by a colorimetric reaction using horse radish peroxidase and 2,2'-azinodi(ethylbenzthiazoline-6-sulfonic acid to measure H2O2 formed during oxidation of amines. The method has a coefficient of variation of approximately 2.5% and provides results comparable with
Antioxidant and cytotoxicological effects of Aloe vera food supplements
Lopez Z, et al.
Journal of Food Quality, 2017 (2017)
Thamizhiniyan Venkatesan et al.
BioMed research international, 2019, 3520675-3520675 (2019-08-31)
It is well established that various extraction factors, including the method, temperature, time, and solvent system, significantly influence the antioxidant quality of plant-derived products. Previously, we observed that extraction of Pinus densiflora bark (PDB) by the most common traditional Soxhlet
Enzyme immunoassay ELISA and EMIT.
E Engvall
Methods in enzymology, 70(A), 419-439 (1980-01-01)
Nike Julia Kräutler et al.
Cell reports, 30(4), 997-1012 (2020-01-30)
Control of established chronic lymphocytic choriomeningitis virus (LCMV) infection requires the production of neutralizing antibodies, but it remains unknown how the ensemble of antibodies evolves during ongoing infection. Here, we analyze the evolution of antibody responses during acute or chronic

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