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4-氯-7-硝基苯并-2-氧杂-1,3-二唑

Name: 4-氯-7-硝基苯并-2-氧杂-1,3-二唑
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

别名:

4-氯-7-硝基-1,2,3-苯并噁二唑, NBD-chloride

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关于此项目

经验公式（希尔记法）:

C₆H₂ClN₃O₃

化学文摘社编号:

10199-89-0

分子量:

199.55

UNSPSC Code:

12352100

NACRES:

NA.32

PubChem Substance ID:

329752738

EC Number:

233-496-4

Beilstein/REAXYS Number:

614212

MDL number:

MFCD00005808

Assay:

≥97.0% (HPLC)

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属性

product line

BioReagent

Quality Segment

100

assay

≥97.0% (HPLC)

mp

97-100 °C, 97-99 °C (lit.)

fluorescence

λ_ex 336 nm in methanol, λ_ex 420 nm; λ_em ~540 nm in ethanol (after derivatization with glycine)

suitability

suitable for fluorescence

SMILES string

[O-][N+](=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

IGHBXJSNZCFXNK-UHFFFAOYSA-N

说明

Application

4-氯-7-硝基苯并-2-氧杂-1,3-二唑（NBD-chloride）是一种荧光检测试剂，用于标记蛋白质中的游离巯基和 N-末端。NBD-chloride 可用作酶抑制剂，以及半胱氨酸硫酸的捕获剂。

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pictograms

GHS07

signalword

Warning

hcodes

H315,H319

pcodes

P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Leslie B Poole et al.

Methods in enzymology, 348, 122-136 (2002-03-12)

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Development of a cell-based nonradioactive glucose uptake assay system for SGLT1 and SGLT2.

Abhinav Kanwal et al.

Analytical biochemistry, 429(1), 70-75 (2012-07-17)

Sodium-dependent glucose cotransporters (SGLT1 and SGLT2), which have a key role in the absorption of glucose in the kidney and/or gastrointestinal tract, have been proposed as a novel therapeutic strategy for diabetes and cardiomyopathy. Here we developed a simple cell-based

Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan: a method to distinguish protein N-terminal acetylation.

Lina F Bernal-Perez et al.

Analytical biochemistry, 428(1), 13-15 (2012-06-09)

A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the

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货号	GTIN
25455-5G	04061825998763
25455-1G	04061825998749
25455-25G	04061825998756