25455
4-氯-7-硝基苯并-2-氧杂-1,3-二唑
BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
别名:
4-氯-7-硝基-1,2,3-苯并噁二唑, NBD-chloride
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关于此项目
经验公式（希尔记法）:
C6H2ClN3O3
化学文摘社编号:
分子量:
199.55
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
EC Number:
233-496-4
Beilstein/REAXYS Number:
614212
MDL number:
产品名称
4-氯-7-硝基苯并-2-氧杂-1,3-二唑, BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
InChI key
IGHBXJSNZCFXNK-UHFFFAOYSA-N
InChI
1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
SMILES string
[O-][N+](=O)c1ccc(Cl)c2nonc12
product line
BioReagent
assay
≥97.0% (HPLC)
mp
97-100 °C
97-99 °C (lit.)
fluorescence
λex 336 nm in methanol
λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)
suitability
suitable for fluorescence
Quality Level
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Application
4-氯-7-硝基苯并-2-氧杂-1,3-二唑（NBD-chloride）是一种荧光检测试剂，用于标记蛋白质中的游离巯基和 N-末端。NBD-chloride 可用作酶抑制剂，以及半胱氨酸硫酸的捕获剂。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
P D Bragg et al.
Biochimica et biophysica acta, 1413(3), 159-171 (1999-11-11)
Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is
Leslie B Poole et al.
Methods in enzymology, 348, 122-136 (2002-03-12)
C165S AhpC in its sulfenate (Cys-SO-) and presumed thiolate (Cys-S-) forms at pH 7 (pKa for sulfenic acid about pH 6.1) exhibit low extinction absorbance bands around 367 and 324 nm, respectively. Sulfenic acid content of the protein can be
Abhinav Kanwal et al.
Analytical biochemistry, 429(1), 70-75 (2012-07-17)
Sodium-dependent glucose cotransporters (SGLT1 and SGLT2), which have a key role in the absorption of glucose in the kidney and/or gastrointestinal tract, have been proposed as a novel therapeutic strategy for diabetes and cardiomyopathy. Here we developed a simple cell-based
Lina F Bernal-Perez et al.
Analytical biochemistry, 428(1), 13-15 (2012-06-09)
A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the
Junjie Yao et al.
NeuroImage, 64, 257-266 (2012-09-04)
We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which
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