biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
9C5, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (341M-94), vial of 0.5 mL concentrate (341M-95), bottle of 1.0 mL predilute (341M-97), vial of 1.0 mL concentrate (341M-96), bottle of 7.0 mL predilute (341M-98)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
isotype
IgG2b
control
hairy cell leukemia
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Quality Level
Gene Information
human ... ACP5(54)
General description
Tartrate resistant acid phosphatase (TRAcP) is a basic, iron-binding protein with high activity towards phosphoproteins, ATP and 4 nitrophenyl phosphate. Expression of TRAcP is reported to be increased in the spleen and monocytes of individuals with Gaucher′s disease, splenocytes and circulating white cells of individuals with hairy cell leukemia, spleens of individuals with Hodgkin disease, and the sera of individuals undergoing active bone turnover. Elevated levels are also reported to be associated with various B-cell and T-cell leukemias and lymphomas, placental decidual cells, syncytiotrophoblasts, and some macrophages distributed throughout maternal and embryonic tissues.
The histochemical identification of hairy cell leukemia via tartrate-resistant acid phosphatase assay has been a standard for over two decades. Anti-TRAcP labels the cells of hairy cell leukemia (HCL) with a high degree of sensitivity and specificity. Worthy also of mention in this regard are anti-annexin A1 and anti-CD11c. Other cells stained with anti-TRAcP are tissue macrophages and osteoclasts, which also express abundant TRAcP activity.
The histochemical identification of hairy cell leukemia via tartrate-resistant acid phosphatase assay has been a standard for over two decades. Anti-TRAcP labels the cells of hairy cell leukemia (HCL) with a high degree of sensitivity and specificity. Worthy also of mention in this regard are anti-annexin A1 and anti-CD11c. Other cells stained with anti-TRAcP are tissue macrophages and osteoclasts, which also express abundant TRAcP activity.
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Analysis Note
 IVD |  IVD |  IVD |  RUO |
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
TRAcP Positive Control Slides, Product No. 341S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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此项目有
A J Janckila et al.
Blood, 85(10), 2839-2844 (1995-05-15)
Tartrate-resistant acid phosphatase (TRAcP) has been an indispensible marker for hairy cell leukemia (HCL) for over two decades. However, the traditional TRAcP cytochemical stain cannot be performed effectively on sections of paraffin-embedded tissues that are important resources for histopathologic evaluation
A J Janckila et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(3), 235-244 (1996-03-01)
We have developed a monoclonal antibody (9C5) for immunohistochemical localization of tartrate-resistant acid phosphatase (TRAcP). This antibody reacts with a denatured epitope of TRAcP and requires enhancement methods to promote antigenicity in paraffin-embedded tissues. We used this antibody to systematically
A J Janckila et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 73(6), 316-324 (1999-01-15)
Tartrate-resistant acid phosphatase (TRAP) is expressed abundantly by osteoclasts and is required for bone resorption. This enzyme is emerging as an important biomarker in bone pathology, both for histochemical identification of osteoclasts and as a serum marker of osteoclast activity
H Yaziji et al.
American journal of clinical pathology, 104(4), 397-402 (1995-10-01)
Immunohistochemical studies were done on formalin-fixed, paraffin-embedded tissues to evaluate the specificity of a newly developed monoclonal antibody (9C5) against tartrate-resistant acid phosphatase. Sections from 195 specimens were examined, which included 33 types of tissues/organs. These tissues included normal, inflammatory
J D Hoyer et al.
American journal of clinical pathology, 108(3), 308-315 (1997-09-18)
The demonstration of tartrate-resistant acid phosphatase (TRAP) activity has long been a cornerstone in the diagnosis of hairy cell leukemia (HCL). Recently a monoclonal antibody to this enzyme has been developed that can be used in an immunoperoxidase method on
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