产品名称
E-钙粘素 人, recombinant, expressed in E. coli, 0.5 mg protein/mL
biological source
human
recombinant
expressed in E. coli
description
0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
sterility
Filtered sterilized solution
assay
≥90% (SDS-PAGE)
form
liquid
packaging
pkg of 100 μg
concentration
0.5 mg protein/mL
technique(s)
cell culture | mammalian: suitable
accession no.
NP_004351.1
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CDH1(999)
Application
上皮钙黏蛋白已作为包被基质蛋白，用于 1）维持长期 ES 或 iPS 细胞培养，然后与适当的 ES 细胞培养基相结合，2）作为包被基质材料，用于极低非特异相互作用的蛋白源 iPS 方案的11R标签重组 TF 细胞内传递，以及 3）作为天然抗原用于抗体生产。
以这些建议作为指导，确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白，用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释，使加入的量均匀覆盖表面。
注：6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔，并在2～10°C 下培养一夜。
3. 培养后，抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干（如果需要保持无菌）。
以这些建议作为指导，确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白，用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释，使加入的量均匀覆盖表面。
注：6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔，并在2～10°C 下培养一夜。
3. 培养后，抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干（如果需要保持无菌）。
Other Notes
MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP
Preparation Note
用密码子优化法构建重组人上皮钙黏蛋白基因（155-710 aa 断片），并以非融合蛋白形式在 大肠杆菌 中表达为包涵体。使用独特的“温度漂移包涵体复性”技术对最终产物进行复性和色谱纯化。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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