Merck
CN

51800C

Sigma-Aldrich

Claycomb 培养基

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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别名:
HL-1 Medium

描述

For research or for further manufacturing use.

质量水平

无菌性

sterile-filtered

形式

liquid

储存条件

protect from light

技术

cell culture | mammalian: suitable

组分

L-glutamine: no

运输

ambient

储存温度

2-8°C

应用

Claycomb培养基是以建立HL-1细胞系的William Claycomb博士命名的,它专用于培养小鼠心肌细胞生长。HL-1是第一个能够在 体外维持分化心肌细胞表型和收缩活性的细胞系。HL-1细胞系可用于研究心肌梗塞后的心肌细胞肥大、测试新型心脏治疗药物和疗法、生产高水平心肌蛋白以及研究成熟心肌细胞特异性基因。补充100 μM去甲肾上腺素、10%胎牛血清(FBS)和4mM L-谷氨酰胺的Claycomb培养基,将能够维持HL-1细胞系和成熟心肌细胞的行为。在光学显微镜下观察HL-1细胞时,可以观察到细胞个体和群体的收缩随心肌细胞逐渐融合而变得更加频繁。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
动植物来源培养基

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Cell death & disease, 11(9), 756-756 (2020-09-17)
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