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Merck
CN

56759

Sigma-Aldrich

Atto 425

BioReagent, suitable for fluorescence

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About This Item

经验公式(希尔记法):
C22H27NO6
分子量:
401.45
UNSPSC代码:
12352108
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

检测方案

≥90% (HPLC)

形式

(Report result)

制造商/商品名称

ATTO-TEC GmbH

透射比

254 nm, >90%
425 nm, >90%

荧光

λex 440 nm; λem 484 nm

λ

in PBS, pH 7.4
in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 423-429 nm Amax

适用性

suitable for fluorescence

运输

wet ice

储存温度

−20°C

SMILES字符串

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(O)=O)c3cc2OC1=O

InChI

1S/C22H27NO6/c1-5-28-20(26)16-10-14-9-15-13(2)12-22(3,4)23(8-6-7-19(24)25)17(15)11-18(14)29-21(16)27/h9-11,13H,5-8,12H2,1-4H3,(H,24,25)

InChI key

WNDDWSAHNYBXKY-UHFFFAOYSA-N

应用

Atto 荧光标记专门设计用于高灵敏度应用,包括单分子检测。Atto 标记具有刚性结构,不显示任何顺反异构化。因此,这些标记物在偶联时显示出极低的光谱偏移和异常的强度。Atto 425 适合于制备荧光标记试剂。

法律信息

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Richard S Agnes et al.
Journal of the American Chemical Society, 132(17), 6075-6080 (2010-04-13)
A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of

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