UKKi020-A

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Klinikum der Universität zu Köln

Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absence
Files showing reprogramming vector expressed or silenced: Image / Result

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes - -
NANOG Yes - -
OCT 4 Yes - -
SSEA-4 Yes - -
FOXD3 Yes -
DNMT3b Yes -
Morphology:

morphology pictures of iPS cell line NP0100-4 before banking and of the thawing control

morphology pictures of iPS cell line NP0100-4 before banking and of the thawing control
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:

expression of NESTIN in differentiated iPS cell line NP0100-4
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:

expression of SOX17 in differentiated iPS cell line NP0100-4
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:

expression of BRACHYURY in differentiated iPS cell line NP0100-4
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 36
Cell line karyotype: Chr.01: Onset of a trisomy (25%)
Karyotyping method: Molecular karyotyping by SNP array
Image / Result

Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: Essential E8^®
Passage Number banked (pre-EBiSC): 34
No. Vials banked (pre-EBiSC): 20

Note: EAUA and CLIP must be completed before order fulfillment

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EBiSC is a trademark of Fraunhofer-Gesellschaft